Mating-type locus control of killer toxins from Kluyveromyces lactis and Pichia acaciae

FEMS Yeast Research
Roland KlassenFriedhelm Meinhardt

Abstract

Killer-toxin complexes produced by Kluyveromyces lactis and Pichia acaciae inhibit cell proliferation of Saccharomyces cerevisiae. Analysis of their actions in haploid MATalpha cells revealed that introduction of the opposite mating-type locus (MATa) significantly suppressed antizymosis. Together with resistance expressed by MATa/MATalpha diploids, the reciprocal action of MATa or MATalpha in haploids of opposite mating types suggests that these killer toxins may be subject to MAT locus control. Congruently, derepressing the silent mating-type loci, HMR and HML, by removing individual components of the histone deacetylase complex Sir1-4, either by transposon-tagging or by chemically inactivating the histone deacetylase catalytic subunit Sir2, yields toxin resistance. Consistent with MAT control of toxin action, killer-toxin-insensitive S. cerevisiae mutants (kti) become mating-compromised despite resisting the toxins' cell-cycle effects. Mating inhibition largely depends on the time point of toxin application to the mating mixtures and is less pronounced in Elongator mutants, whose resistance to the toxins' cell-cycle effects is the result of toxin-target process deficiencies. In striking contrast, non-Elongator mutants defecti...Continue Reading

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Citations

May 8, 2007·Applied and Environmental Microbiology·John P PaluszynskiFriedhelm Meinhardt
Sep 27, 2013·PloS One·Megumi ShigematsuHaruhiko Masaki
Jun 6, 2008·Molecular Microbiology·Roland KlassenFriedhelm Meinhardt
Nov 23, 2007·Biochemical Society Transactions·D Jablonowski, R Schaffrath

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