Matrine inhibits prostate cancer via activation of the unfolded protein response/endoplasmic reticulum stress signaling and reversal of epithelial to mesenchymal transition

Molecular Medicine Reports
Junli ChangYanping Yang

Abstract

Prostate cancer is the second most commonly diagnosed malignancy and the sixth global primary cause of malignancy‑associated fatality. Increased invasiveness and motility in prostate cancer cells are associated with ubiquitin proteasome system‑regulated epithelial to mesenchymal transition (EMT). Impairment of the endoplasmic reticulum (ER) causes ER stress due to the accumulation of unfolded proteins and altered cell survival. In the current study, the effect and mechanism of matrine on cell apoptosis, viability, migration and invasion of human prostate cancer cells in vivo and in vitro through the unfolded protein response (UPR)/ER stress pathway were investigated. Matrine inhibited proteasomal chymotrypsin‑like (CT‑like) activity in the prostate carcinoma cellular proteasome. Upregulated vimentin and N‑cadherin and downregulated E‑cadherin were also observed in vitro and in vivo. In vitro analyses showed that matrine repressed cell motility, viability and invasion, arrested the cell cycle at the G0/G1 phase and induced prostate cancer cell apoptosis. Furthermore, matrine activated the UPR/ER stress signaling cascade in prostate cancer cells and tumor tissues of xenograft‑bearing nude mice. Results also demonstrated that the ...Continue Reading

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Citations

Feb 29, 2020·Cancer Management and Research·Linlin LiKaichen Wang
Mar 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hai ShangZhongmei Zou
May 11, 2019·Journal of Clinical Medicine·Patricia G SantamaríaFrancisco Portillo
Jun 2, 2020·Frontiers in Pharmacology·Hong ZhangCheng Guo

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Methods Mentioned

BETA
flow cytometry
fluorescence microscopy
xenograft
xenografts
PCR
protein folding

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