Abstract
Matrine (MAT), a quinolizidine alkaloid derived from the herb Radix Sophorae flavescens, has been recently found to be beneficial in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, mainly through its anti-inflammatory effect. In the present study, we tested the effect of MAT on ongoing EAE and defined possible mechanisms underlying its effects on myelination and oligodendrocytes. EAE was induced in C57BL/6 mice and MAT treatment was started at disease onset. Clinical scores were monitored daily; spinal cords and the corpus callosum brain region of mice were harvested on day 23 p.i. for inflammatory infiltration and demyelination of the central nervous system. Myelin content and the development of oligodendrocytes and their precursors were determined by immunostaining, and expression of p-Akt, p-mTOR, p-PI3K, and p-P70S6 was determined by Western blot. MAT effectively suppressed EAE severity and increased the expression of proteolipid protein, a myelin protein that is a marker of CNS myelin. MAT treatment largely increased the number of mature oligodendrocytes, and significantly activated the PI3K/Akt/mTOR signaling pathway, which is required for oligodendrocyte survival and axon myelinati...Continue Reading
Citations
Feb 13, 2018·Frontiers in Pharmacology·Bo ZhangShao Li
Jan 14, 2020·Molecular Neurobiology·Vera PaschonAlexandre Hiroaki Kihara
Sep 11, 2019·Journal of Separation Science·Xin DongBaoQuan Bao
Jun 2, 2020·Frontiers in Pharmacology·Hong ZhangCheng Guo
Apr 19, 2018·The International Journal of Neuropsychopharmacology·Zhonghua WuHui Xu
Oct 13, 2020·Frontiers in Pharmacology·Longtai YouJian Ni
Mar 12, 2021·Brain Research·Zhe DingLijuan Lin
Dec 12, 2018·International Immunopharmacology·Shuangwen YuKaili Hu
Apr 30, 2021·Frontiers in Immunology·Mengmeng DouLin Zhu
May 4, 2021·Journal of Neuroimmunology·Huiqing HouXiujuan Song
May 6, 2021·Scientific Reports·Jian KangLin Zhu
Jul 10, 2021·Frontiers in Cellular Neuroscience·Han LiuYuming Xu
Jul 20, 2021·Journal of Chemical Neuroanatomy·Yilei JingLin Zhu
Aug 15, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sajad FakhriJianbo Xiao
Sep 15, 2020·Journal of Agricultural and Food Chemistry·Chunhong LiuHans-Christian Siebert