Aug 8, 2008

Matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients

Journal of Clinical Microbiology
Nicolas DegandAgnes Ferroni

Abstract

The identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (CF) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. These techniques remain time-consuming, expensive, and technically demanding. We used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) for the identification of these bacteria. A set of reference strains belonging to 58 species of clinically relevant nonfermenting gram-negative bacilli was used. To identify peaks discriminating between these various species, the profile of 10 isolated colonies obtained from 10 different passages was analyzed for each referenced strain. Conserved peaks with a relative intensity greater than 0.1 were retained. The spectra of 559 clinical isolates were then compared to that of each of the 58 reference strains as follows: 400 Pseudomonas aeruginosa, 54 Achromobacter xylosoxidans, 32 Stenotrophomonas maltophilia, 52 Burkholderia cepacia complex (BCC), 1 Burkholderia gladioli, 14 Ralstonia mannitolilytica, 2 Ralstonia pickettii, 1 Bordetella hinzii, 1 Inquilinus limosus, 1 Cupriavidus respiraculi, and 1 Burkholderia thailandensis. Using this database, 54...Continue Reading

  • References23
  • Citations102

References

  • References23
  • Citations102

Citations

Mentioned in this Paper

Surface Enhanced Laser Desorption Ionization Mass Spectrometry
Cupriavidus respiraculi
Pseudomonas aeruginosa (antigen)
Burkholderia Infections
Ralstonia pickettii
Bordetella hinzii
Cystic Fibrosis
Burkholderia thailandensis
Mass Spectrometry
Gram-Negative Bacteria

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