Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based method for discrimination between molecular types of Cryptococcus neoformans and Cryptococcus gattii.

Journal of Clinical Microbiology
Brunella PosteraroA M Tortorano

Abstract

We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.

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Citations

May 10, 2013·Journal of Clinical Microbiology·Lars F WestbladeCarey-Ann D Burnham
Jul 5, 2013·Journal of Clinical Microbiology·Xiaobo FengZhirong Yao
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