Matrix-assisted laser desorption ionization-time of flight and comparative genomic analysis of M-18 group a Streptococcus strains associated with an acute rheumatic fever outbreak in northeast Italy in 2012 and 2013

Journal of Clinical Microbiology
Paolo GaibaniMaria Paola Landini

Abstract

Acute rheumatic fever (ARF) is a postsuppurative sequela caused by Streptococcus pyogenes infections affecting school-age children. We describe here the occurrence of an ARF outbreak that occurred in Bologna province, northeastern Italy, between November 2012 and May 2013. Molecular analysis revealed that ARF-related group A Streptococcus (GAS) strains belonged to the M-18 serotype, including subtypes emm18.29 and emm18.32. All M-18 GAS strains shared the same antigenic profile, including SpeA, SpeB, SpeC, SpeL, SpeM, and SmeZ. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analysis revealed that M-18 GAS strains grouped separately from other serotypes, suggesting a different S. pyogenes lineage. Single nucleotide polymorphisms and phylogenetic analysis based on whole-genome sequencing showed that emm18.29 and emm18.32 GAS strains clustered in two distinct groups, highlighting genetic variations between these subtypes. Comparative analysis revealed a similar genome architecture between emm18.29 and emm18.32 strains that differed from noninvasive emm18.0 strains. The major sources of differences between M-18 genomes were attributable to the prophage elements. Prophage regions contained several virulence f...Continue Reading

References

Apr 1, 1996·Journal of Clinical Microbiology·B BeallT Thompson
Jul 6, 2000·Pediatrics·M C McCormick, M A Stoto
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·James C SmootJames M Musser
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stephen B BeresJames M Musser
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Carlos CanchayaHarald Brüssow
Oct 3, 2006·Journal of Bacteriology·Matthew T G HoldenJulian Parkhill
Sep 26, 2009·The Lancet Infectious Diseases·Andrew C SteerJonathan R Carapetis
Dec 17, 2009·Nature Reviews. Microbiology·Sascha Sauer, Magdalena Kliem
Apr 8, 2010·Science Translational Medicine·Ramy K Aziz, Victor Nizet
Sep 8, 2010·Nucleic Acids Research·Israel S FernándezOscar Llorca
Aug 10, 2011·BMC Genomics·Nabil-Fareed AlikhanScott A Beatson
Oct 27, 2011·Genome Biology·Julian Parkhill, Brendan W Wren
Sep 1, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A FriãesJ Melo-Cristino
Apr 4, 2014·Clinical Microbiology Reviews·Mark J WalkerVictor Nizet
Jun 4, 2014·International Reviews of Immunology·Madeleine W Cunningham
Aug 15, 2014·Frontiers in Genetics·Mick Watson
Aug 15, 2014·The Journal of Biological Chemistry·Nina N SchommerRichard L Gallo
Oct 1, 2014·The Journal of Biological Chemistry·Anna HenninghamJason N Cole

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Mar 31, 2017·Journal of Clinical Microbiology·Jennifer E CornickDean B Everett

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