Matrix-assisted laser desorption/ionization mass spectrometric peptide mapping of high molecular weight glutenin subunits 1Bx7 and 1Dy10 in Cheyenne cultivar

Rapid Communications in Mass Spectrometry : RCM
R CozzolinoA Palermo

Abstract

This study describes the verification of the cDNA-deduced amino acid sequences of high molecular weight glutenin subunits 1Dy10 and 1Bx7 in Cheyenne cultivar by direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) analysis of their tryptic fragments omitting chromatographic pre-separation. These polypeptides have a conserved structure consisting of a long central repetitive domain that prevents the application of conventional sequencing procedures such as Edman degradation. The published sequence of subunit 1Dy10 contains 7 Lys and 13 Arg residues; thus the production of 21 tryptic peptides is expected. The cDNA-deduced sequence for 1Bx7 subunit includes 5 Lys and 15 Arg residues, but the presence of three Arg-Pro bonds, which are normally not cleaved by trypsin, predicts only 19 tryptic peptides. Three different matrices (DHB, SA and HCCA) in combination with the most compatible sample preparation procedures were used in order to obtain the maximum 1Dy10 and 1Bx7 sequence coverage. MALDI analysis of the 1Dy10 tryptic digest resulted in the identification of all 21 expected peptides. In the case of 1Bx7 MALDI analysis resulted in the identification of 17 of the 19 expected peptides, ...Continue Reading

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Citations

Nov 16, 2001·Phytochemical Analysis : PCA
Jun 16, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·X AnF J Zeller
Oct 18, 2011·Journal of Agricultural and Food Chemistry·Vera MuccilliSalvatore Foti
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Jul 1, 2005·Rapid Communications in Mass Spectrometry : RCM·Gaetano AlberghinaAnna Savarino
Oct 16, 2009·Journal of Mass Spectrometry : JMS·Gianfranco MamonePasquale Ferranti
Sep 17, 2013·Journal of Proteome Research·Miguel RibeiroGilberto Igrejas
Jul 11, 2001·Rapid Communications in Mass Spectrometry : RCM·R CozzolinoA Palermo
Oct 13, 2001·Journal of Mass Spectrometry : JMS
Jun 20, 2012·Mass Spectrometry Reviews·Vincenzo CunsoloSalvatore Foti
Jun 10, 2004·European Journal of Mass Spectrometry·Vincenzo CunsoloRosaria Saletti
Aug 29, 2006·Genome Génome / Conseil National De Recherches Canada·Yanzhen ZhangFriedrich J Zeller
Jul 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·You-Ran JangJong-Yeol Lee
Sep 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·You-Ran JangJong-Yeol Lee

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