Matrix-assisted laser desorption/ionization mass spectrometric analysis of uncomplexed highly sulfated oligosaccharides using ionic liquid matrices

Analytical Chemistry
Tatiana N LaremoreRobert J Linhardt

Abstract

Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulfated oligosaccharides do not produce any signal. We report that 1-methylimidazolium alpha-cyano-4-hydroxycinnamate and butylammonium 2,5-dihydroxybenzoate ionic liquid matrices allow the detection of picomole amounts of the sodium salts of a disaccharide, sucrose octasulfate, and an octasulfated pentasaccharide, Arixtra. The experimental results indicate that both analytes undergo some degree of thermal fragmentation with a mass loss corresponding to cleavage of O-SO3Na bonds in the matrix upon laser irradiation, reflecting lability of sulfo groups.

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Citations

Jul 11, 2006·Analytical and Bioanalytical Chemistry·Andreas Tholey, Elmar Heinzle
Nov 7, 2006·Analytical and Bioanalytical Chemistry·Chensong PanHanfa Zou
Oct 30, 2008·Analytical and Bioanalytical Chemistry·Rui LiuGui-bin Jiang
Aug 19, 2009·Analytical and Bioanalytical Chemistry·Nils HellwigJürgen Grotemeyer
Jul 8, 2010·Analytical and Bioanalytical Chemistry·Estela GiménezVictoria Sanz-Nebot
Sep 21, 2010·Analytical and Bioanalytical Chemistry·Bo YangRobert J Linhardt
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Rongrong HuangJoshua S Sharp
Jul 25, 2012·Journal of the American Society for Mass Spectrometry·Franklin E LeachI Jonathan Amster
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