Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups

Rapid Communications in Mass Spectrometry : RCM
Jong-Seo KimHie-Joon Kim

Abstract

Picolinamidination of amino groups in peptides was carried out using ethyl picolinimidate tetrafluoroborate synthesized from picolinamide and triethyloxonium tetrafluoroborate. The N-terminal amino group as well as the epsilon-amino group of lysine were derivatized. The matrix-assisted laser desorption/ionization (MALDI) signal of a peptide was enhanced 20-35-fold upon picolinamidination depending on the number of amino groups derivatized. The signal enhancement effect is much higher than that of acetamidination or guanidination previously reported. Improved protein identification by mass mapping of the derivatized peptides was demonstrated.

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Citations

Jul 10, 2009·Journal of the American Society for Mass Spectrometry·Jong-Seo KimHie-Joon Kim
Nov 23, 2016·The Analyst·Mahalia A C SerranoS Thayumanavan
Apr 6, 2019·Journal of the American Society for Mass Spectrometry·Kenneth N RobinsonJosephine Bunch
Dec 28, 2011·Analytical and Bioanalytical Chemistry·Jong-Seo KimHie-Joon Kim

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