Matrix-assisted laser desorption/ionization imaging mass spectrometry for the investigation of proteins and peptides

Annual Review of Analytical Chemistry
Kristin E BurnumRichard M Caprioli

Abstract

Mass spectrometry (MS) is an excellent technology for molecular imaging because of its high data dimensionality. MS can monitor thousands of individual molecular data channels measured as mass-to-charge (m/z). We describe the use of matrix-assisted laser desorption/ionization (MALDI) MS for the image analysis of proteins, peptides, lipids, drugs, and metabolites in tissues. We discuss the basic instrumentation and sample preparation methods needed to produce high-resolution images and high image reproducibility. Matrix-addition protocols are briefly discussed along with normal operating procedures, and selected biological and medical applications of MALDI imaging MS are described. We give examples of both two- and three-dimensional imaging, including normal mouse embryo implantation, sperm maturation in mouse epididymis, protein distributions in brain sections, protein alterations as a result of drug administration, and protein changes in brain due to neurodegeneration and tumor formation. Advantages of this technology and future challenges for its improvement are discussed.

References

Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jul 2, 2003·Pharmacogenomics·Wolfgang PuschMarkus Kostrzewa
Nov 5, 2003·Proteomics·Pierre ChaurandRichard M Caprioli
Feb 14, 2004·Analytical Chemistry·Pierre ChaurandRichard M Caprioli
Apr 20, 2004·Toxicology Letters·Jennie L Walgren, David C Thompson
Apr 29, 2004·Journal of Proteome Research·György Marko-Varga, Thomas E Fehniger
May 29, 2004·Neurobiology of Aging·Bärbel SchönheitThomas G Ohm
Oct 7, 2004·The American Journal of Pathology·Pierre ChaurandRichard M Caprioli
Nov 13, 2004·Drug Discovery Today·David A Lewin, Michael P Weiner
Dec 22, 2004·Mechanisms of Ageing and Development·Tatiana C RohnerMarkus Stoeckli
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·Peter R Bieck, William Z Potter
May 25, 2005·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Markus RudinMarkus Stoeckli
Jun 1, 2005·Journal of the American Society for Mass Spectrometry·Anna C CreceliusBobby Bodenheimer
Dec 16, 2005·Pharmacogenomics·Kathryn J StrandMeena Augustus
Feb 2, 2006·Analytical Chemistry·Hans-Rudolf AerniRichard M Caprioli
Feb 7, 2006·Journal of Proteome Research·Karl SköldPer E Andrén
Aug 22, 2006·Journal of Pharmacological and Toxicological Methods·Yunsheng HsiehWalter A Korfmacher
Sep 8, 2006·Journal of Neuropathology and Experimental Neurology·Mahlon D JohnsonRichard M Caprioli
Sep 15, 2006·Analytical Chemistry·Sheerin Khatib-ShahidiRichard M Caprioli
Oct 24, 2006·Journal of the American Society for Mass Spectrometry·Ioana M TabanGökhan Baykut
Dec 13, 2006·Nature Reviews. Drug Discovery·Alexander KambChristoph Lengauer
Jan 6, 2007·Journal of Proteome Research·Bruno MeunierJean-François Hocquette
Jan 16, 2007·Analytical Chemistry·Yue LiAkos Vertes
Jan 19, 2007·Journal of Mass Spectrometry : JMS·M Reid GrosecloseRichard M Caprioli
Feb 13, 2007·Journal of Proteome Research·R LemaireI Fournier
Mar 27, 2007·Journal of Proteome Research·Stephanie S DeKeyserLingjun Li
Jun 2, 2007·International Journal of Mass Spectrometry·Jeremy L NorrisRichard M Caprioli
Jul 11, 2007·Journal of Mass Spectrometry : JMS·John A McLeanRichard M Caprioli
Jul 28, 2007·Journal of Proteome Research·Pierre LescuyerThierry Rabilloud
Jul 31, 2007·Analytical Chemistry·Dodge L BaluyaRichard A Yost
Jan 1, 2008·Nature Methods·Malin AnderssonRichard M Caprioli
Feb 1, 2006·Personalized Medicine·Peter Oehr

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Citations

Dec 29, 2010·Analytical and Bioanalytical Chemistry·Maria Careri, Alessandro Mangia
Oct 29, 2010·Histochemistry and Cell Biology·Jaroslav PólMichael Volný
Apr 18, 2013·Journal of the American Society for Mass Spectrometry·Andrew R Korte, Young Jin Lee
Apr 30, 2010·Journal of the American Society for Mass Spectrometry·Marion GirodR Graham Cooks
Aug 24, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Mohammadreza ShariatgorjiPer E Andrén
Jun 4, 2010·The Analyst·Carol E ParkerChristoph H Borchers
Jul 20, 2010·Annual Review of Analytical Chemistry·Mélanie AbonnencHubert H Girault
Dec 18, 2008·Annual Review of Biophysics·Steven G BoxerPeter K Weber
Feb 18, 2011·The AAPS Journal·Parimal Samir, Andrew J Link
Apr 25, 2014·Journal of the American Society for Mass Spectrometry·Roberto FernándezJosé A Fernández
Apr 20, 2014·Journal of the American Society for Mass Spectrometry·Jeremy A BarryDavid C Muddiman
Jun 29, 2010·Trends in Biotechnology·Ales Svatos
Apr 5, 2011·Proteomics·Stephanie KasparHans-Peter Mock
Nov 19, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Domenico TavernaRichard Caprioli
Jan 17, 2012·Proteomics·Christof Rampitsch, Natalia V Bykova
Sep 29, 2015·Analytical Chemistry·Elias P RosenDavid C Muddiman
Apr 5, 2015·Journal of the American Society for Mass Spectrometry·Elias P RosenDavid C Muddiman
Jul 19, 2008·Annual Review of Analytical Chemistry·Lingjun Li, Jonathan V Sweedler
May 16, 2014·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·I MillerA C Gutleb
Nov 13, 2015·International Journal of Molecular Sciences·Zahed HossainSetsuko Komatsu
Oct 19, 2017·Expert Review of Proteomics·Tadashi Kondo
Sep 17, 2013·Angewandte Chemie·Shane R EllisRon M A Heeren
Jul 28, 2018·Journal of Lipid Research·Estefanía Moreno-GordalizaM Milagros Gómez-Gómez
Oct 24, 2018·Expert Review of Proteomics·Cristina HerreraJosé María Gutiérrez
Nov 26, 2009·Natural Product Reports·Eduardo EsquenaziPieter C Dorrestein
Jan 14, 2010·Rapid Communications in Mass Spectrometry : RCM·D C PerdianR S Houk
May 2, 2018·Arab Journal of Urology·Manesh Kumar Panner Selvam, Ashok Agarwal
Mar 9, 2019·Annual Review of Analytical Chemistry·Ian S GilmoreCornelius L Pieterse
Feb 21, 2019·Annual Review of Analytical Chemistry·Luke HanleyYeni P Yung
Jun 9, 2012·The Analyst·Bojidarka Ivanova, Michael Spiteller
Dec 22, 2019·Antioxidants & Redox Signaling·Ashok AgarwalKruyanshi Master
Nov 18, 2011·Expert Review of Proteomics·Juan J Calvete
Dec 19, 2012·Molecular Human Reproduction·Julius NyalwidheSergio Oehninger
Dec 17, 2010·Chemical Communications : Chem Comm·Allison L DillR Graham Cooks
Jan 14, 2020·Pharmacology & Therapeutics·Eiji SugiyamaYuki Sugiura
Jan 22, 2017·Talanta·Estefanía Moreno-GordalizaM Milagros Gómez-Gómez
Aug 5, 2010·Analytical Chemistry·Julia BalogZoltan Takats

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Methods Mentioned

BETA
biopsies
imaging techniques
confocal microscopy

Software Mentioned

SAM

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