Matrix Deformation with Ectopic Cells Induced by Rotational Motion in Bioengineered Neural Tissues.

Annals of Biomedical Engineering
Nicolas RouleauDavid L Kaplan

Abstract

The brain's extracellular matrix (ECM) is a dynamic protein-based scaffold within which neural networks can form, self-maintain, and re-model. When the brain incurs injuries, microscopic tissue tears and active ECM re-modelling give way to abnormal brain structure and function including the presence of ectopic cells. Post-mortem and neuroimaging data suggest that the brains of jet pilots and astronauts, who are exposed to rotational forces, accelerations, and microgravity, display brain anomalies which could be indicative of a mechanodisruptive pathology. Here we present a model of non-impact-based brain injury induced by matrix deformation following mechanical shaking. Using a bioengineered 3D neural tissue platform, we designed a repetitive shaking paradigm to simulate subtle rotational acceleration. Our results indicate shaking induced ectopic cell clustering that could be inhibited by physically restraining tissue movement. Imaging revealed that the collagen substrate surrounding cells was deformed following shaking. Applied to neonatal rat brains, shaking induced deformation of extracellular spaces within the cerebral cortices and reduced the number of cell bodies at higher accelerations. We hypothesize that ECM deformatio...Continue Reading

References

May 1, 1976·Archives of Environmental Health·R C SpearT H Milby
Aug 1, 1968·Journal of Neurology, Neurosurgery, and Psychiatry·D R Oppenheimer
Jun 27, 2000·Biophysical Journal·C M LoY L Wang
Nov 16, 2002·Archives of Neurology·Philip J Horner, Fred H Gage
Jun 2, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lei WangMichael Chopp
Jan 31, 2007·Stroke; a Journal of Cerebral Circulation·Bing-Qiao ZhaoEng H Lo
Mar 27, 2007·Annals of Biomedical Engineering·D Kacy CullenMichelle C LaPlaca
Jul 23, 2011·Science·Rosa C PaolicelliCornelius T Gross
Jun 8, 2012·Journal of the Royal Society, Interface·Erik H ClaytonPhilip V Bayly
Sep 6, 2012·Aviation, Space, and Environmental Medicine·Daekeon LimSeungcheol Kang
Mar 5, 2013·Journal of Science and Medicine in Sport·Jesse MuirClinton T Rubin
Nov 21, 2013·Acta Neuropathologica Communications·Miguel A Gama SosaGregory A Elder
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Min D Tang-SchomerDavid L Kaplan
Dec 17, 2014·Frontiers in Cellular Neuroscience·Noelia Urbán, François Guillemot
Jan 6, 2015·Journal of Neuroscience Research·Ji-Geng YanRobert Havlik
Jun 13, 2015·Journal of the Royal Society, Interface·Kaveh LaksariDavid C Camarillo
Jun 25, 2015·Journal of the American Heart Association·Joanna M WardlawSusana Muñoz-Maniega
Aug 14, 2015·Nature Protocols·Karolina ChwalekDavid L Kaplan
Oct 5, 2015·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Ji-Geng YanRobert Havlik
Jan 19, 2018·Journal of Neuroscience Research·Naijil George, Herbert M Geller
Feb 18, 2020·Macromolecular Bioscience·Nicolas RouleauDavid L Kaplan
Dec 10, 2018·ACS Biomaterials Science & Engineering·William CantleyDavid L Kaplan

❮ Previous
Next ❯

Software Mentioned

SPSS
Elements
NIS
ImageJ

Related Concepts

Related Feeds

Biomaterial Engineering

Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Related Papers

Occupational Medicine
John Hobson
The Lancet. Haematology
The Lancet Haematology
Journal of Forensic Sciences
M G Gilliland, R Folberg
Science
Jennifer Couzin-Frankel
© 2021 Meta ULC. All rights reserved