Matrix-free laser desorption/ionization mass spectrometry using silicon glancing angle deposition (GLAD) films

Rapid Communications in Mass Spectrometry : RCM
Abebaw B JemereD Jed Harrison

Abstract

Glancing angle deposition (GLAD) was used to fabricate nanostructured silicon (Si) thin films with highly controlled morphology for use in laser desorption/ionization mass spectrometry (DIOS-MS). Peptides, drugs and metabolites in the mass range of 150-2500 Da were readily analyzed. The best performance was obtained with 500 nm thick films deposited at a deposition angle of 85 degrees . Low background mass spectra and attomole detection limits were observed with DIOS-MS for various peptides. Films used after three months of dry storage in ambient conditions produced mass spectra with negligible low-mass noise following a 15 min UV-ozone treatment. The performance of the Si GLAD films was as good as or better than that reported for electrochemically etched porous silicon and related materials, and was superior to matrix-assisted laser desorption/ionization (MALDI)-MS for analysis of mixtures of small molecules between 150-2500 Da in terms of background chemical noise, detection limits and spot-to-spot reproducibility. The spot-to-spot reproducibility of signal intensities (100 shots/spectrum) from 21 different Si GLAD film targets was +/-13% relative standard deviation (RSD). The single shot-to-shot reproducibility of signals on...Continue Reading

References

Sep 11, 2002·Rapid Communications in Mass Spectrometry : RCM·Victor V LaikoVladimir M Doroshenko
Nov 17, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Shufen TsoiJonathan G C Veinot
Mar 15, 2005·Analytical Chemistry·E P GoG Siuzdak
Aug 16, 2005·Analytical Chemistry·Shoji OkunoYoshinao Wada
Sep 13, 2006·Mass Spectrometry Reviews·Dominic S Peterson
Mar 9, 2007·Journal of Proteome Research·Eden P GoGary Siuzdak
Oct 26, 2007·Nature·Trent R NorthenGary Siuzdak
Feb 8, 2008·Journal of Chromatography. a·Louis W Bezuidenhout, Michael J Brett
Dec 17, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Kathleen M KrauseMichael J Brett
Jan 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Gaëlle PiretRabah Boukherroub

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