Matrix GLA protein function in human trabecular meshwork cells: inhibition of BMP2-induced calcification process

Investigative Ophthalmology & Visual Science
Wei XueTeresa Borrás

Abstract

The matrix GLA (MGP) gene has been found to be among the 10 most highly expressed genes in the human trabecular meshwork (TM), and its expression is affected by conditions associated with glaucoma. Because MGP protein has been shown to play a key role in inhibiting calcification in cartilage and arterial vessels, MGP's function in human TM was investigated. Perfused TM tissue and primary human TM (HTM) cells originated from donors of nonglaucomatous eyes. MGP mRNA was assayed by relative quantitative and real-time PCR. AdhMGP recombinant adenovirus was generated by bacterial transposition. Western blot analyses were cross-reacted with MGP N-terminal- and conformational-specific antibodies. MGP/BMP2 colocalization was analyzed by confocal microscopy. gamma-Carboxylation activity was measured by incorporation of 14CO2 into FLEEL synthetic peptide. Alkaline phosphatase (ALP) activity was used as a marker of osteogenic differentiation and a calcification precursor. Calcification was assessed by measuring direct calcium (o-cresolphthalein). Normalization was conducted with a telomerase probe (genomic DNA). HTM cells contained high levels of gamma-carboxylase activity and were able to convert MGP to its active conformation. Overexpre...Continue Reading

References

Dec 28, 1983·Biochemical and Biophysical Research Communications·P A PriceY Otawara
Sep 22, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·P A PriceM K Williamson
Feb 17, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·P A PriceM K Williamson
Jun 9, 2000·Kidney International·P Ducy, G Karsenty
Jun 16, 2001·Journal of Cellular Biochemistry·E A OlmstedA R Davis
Nov 1, 2001·Annual Review of Cell and Developmental Biology·R L Chow, R A Lang
Mar 6, 2003·Thrombosis Research·Reidar WallinSusan M Hutson
May 27, 2003·Investigative Ophthalmology & Visual Science·Stanislav I TomarevIrina Malyukova
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·A SweattR Wallin
Sep 2, 2003·Cytokine & Growth Factor Reviews·Mohamad AzharThomas Doetschman
Oct 31, 2003·Journal of Cellular Biochemistry·Amina F ZebboudjKristina Boström
Mar 20, 2004·Journal of Vascular Research·Victoria ShinKristina Boström
Jul 29, 2004·Journal of Cellular Physiology·Jason Vittitow, Teresa Borrás
Apr 6, 2005·American Journal of Medical Genetics. Part a·David J HurSimeon A Boyadjiev
Jun 15, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D MagneJ Guicheux

❮ Previous
Next ❯

Citations

Feb 22, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Mony ShuvyChaim Lotan
Apr 30, 2009·Physiological Genomics·Núria Comes, Teresa Borrás
Jun 27, 2007·Investigative Ophthalmology & Visual Science·Wei XueTeresa Borrás
Jul 4, 2013·Investigative Ophthalmology & Visual Science·Lakisha K BuieTeresa Borrás
May 23, 2012·PloS One·K David KennedyTeresa Borrás
Feb 26, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Teresa Borrás
May 9, 2014·Biotechnology Advances·Cula N DautricheSusan T Sharfstein
Jan 15, 2013·Advances in Medical Sciences·H Ayari, G Bricca
Jan 3, 2012·Experimental Eye Research·K M Perkumas, W D Stamer
Dec 17, 2008·Experimental Eye Research·Teresa Borrás, Núria Comes
Jul 31, 2007·Progress in Retinal and Eye Research·Renata PiccianiSanjoy K Bhattacharya
Mar 16, 2007·Experimental Eye Research·W Daniel StamerC Ross Ethier
May 31, 2015·Experimental Eye Research·Iok-Hou PangAbbot F Clark
May 17, 2008·Molecular Ecology·Erik AxelssonHans Ellegren
Sep 12, 2014·International Journal of Cell Biology·Satoru Ken Nishimoto, Miyako Nishimoto
Mar 4, 2008·Experimental Eye Research·Ted S Acott, Mary J Kelley
Jun 1, 2016·Ageing Research Reviews·Sergio Claudio SaccàAlberto Izzotti
Jul 28, 2016·Experimental Eye Research·Ilya RybkinJohn Danias
Sep 7, 2016·Experimental Eye Research·Teresa Borrás
Apr 7, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Olivier M VanakkerAnne De Paepe
Mar 29, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Chao WangNils A Loewen
Dec 26, 2018·Frontiers in Cardiovascular Medicine·David NgaiMichelle P Bendeck
Apr 5, 2018·Scientific Reports·Yalong DangNils A Loewen
May 21, 2021·Translational Vision Science & Technology·Wenyan WangWei Zhu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.