Matrix immobilization enhances the tissue repair activity of growth factor gene therapy vectors

Human Gene Therapy
John DoukasG F Pierce

Abstract

Although growth factor proteins display potent tissue repair activities, difficulty in sustaining localized therapeutic concentrations limits their therapeutic activity. We reasoned that enhanced histogenesis might be achieved by combining growth factor genes with biocompatible matrices capable of immobilizing vectors at delivery sites. When delivered to subcutaneously implanted sponges, a platelet-derived growth factor B-encoding adenovirus (AdPDGF-B) formulated in a collagen matrix enhanced granulation tissue deposition 3- to 4-fold (p < or = 0.0002), whereas vectors encoding fibroblast growth factor 2 or vascular endothelial growth factor promoted primarily angiogenic responses. By day 8 posttreatment of ischemic excisional wounds, collagen-formulated AdPDGF-B enhanced granulation tissue and epithelial areas up to 13- and 6-fold (p < 0.009), respectively, and wound closure up to 2-fold (p < 0.05). At longer times, complete healing without excessive scar formation was achieved. Collagen matrices were shown to retain both vector and transgene products within delivery sites, enabling the transduction and stimulation of infiltrating repair cells. Quantitative PCR and RT-PCR demonstrated both vector DNA and transgene mRNA within ...Continue Reading

References

Jan 1, 1991·Annual Review of Medicine·T F DeuelA F Pierce
Jan 1, 1990·Annals of Plastic Surgery·S T Ahn, T A Mustoe
Aug 1, 1989·The Journal of Clinical Investigation·S E LynchH N Antoniades
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A BuckleyS C Woodward
Dec 1, 1985·The Journal of Clinical Investigation·G R GrotendorstA K Harvey
Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·J GerdesH Stein
Mar 1, 1994·The Journal of Experimental Medicine·G F PierceC F Morris
Nov 1, 1994·The Journal of Clinical Investigation·R K KhouriG F Pierce
Jan 1, 1995·Annual Review of Medicine·G F Pierce, T A Mustoe
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·P M VogtE Eriksson
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·C AndreeE Eriksson
Jan 1, 1996·The Journal of Cell Biology·J Xu, R A Clark
May 2, 1996·The New England Journal of Medicine·J M Wilson
Jul 1, 1996·The Journal of Trauma·D G Greenhalgh
Dec 15, 1996·The Journal of Clinical Investigation·S I BennJ M Davidson
Oct 20, 1998·American Journal of Surgery·D L Steed
Oct 20, 1998·American Journal of Surgery·T J Wieman
Mar 20, 1999·The Journal of Investigative Dermatology·S A EmingJ M Davidson
Jul 30, 1999·Nature Medicine·C Nyberg-Hoffman, E Aguilar-Cordova
Jul 31, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J DoukasB A Sosnowski
Sep 1, 1999·The Journal of Investigative Dermatology·K W LiechtyT M Crombleholme
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Aug 19, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·L A ChandlerG F Pierce
Feb 24, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·L A ChandlerM L Phillips
Oct 1, 1994·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·T A MustoeG F Pierce

❮ Previous
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Citations

Nov 20, 2002·The Journal of Gene Medicine·Franz SchererChristian Plank
Jun 23, 2006·Der Unfallchirurg·A OberholzerW Ertel
Mar 3, 2007·Der Orthopäde·S K Tschöke, A Oberholzer
Apr 4, 2007·Pharmaceutical Research·Mohamed HaiderKam W Leong
Jan 28, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zaki MegeedHamidreza Ghandehari
Mar 12, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohamed HaiderHamidreza Ghandehari
Apr 20, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Qiming JinWilliam V Giannobile
Jan 26, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Courtney A DunnWilliam V Giannobile
Jan 7, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Seungjin Shin, Lonnie D Shea
Jun 2, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jae-Hyung JangLonnie D Shea
Sep 19, 2003·Tissue Engineering·Orasa AnusaksathienWilliam V Giannobile
Jun 10, 2009·Tissue Engineering. Part C, Methods·Michael KeeneyAbhay Pandit
Sep 24, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Danling GuMonica L Zepeda
Feb 8, 2002·American Journal of Physiology. Cell Physiology·Qi-Ping Chen, William V Giannobile
Jan 1, 2009·Journal of Indian Society of Periodontology·Swapna MahaleTriveni Kale
Sep 1, 2004·Expert Opinion on Biological Therapy·Sabine A EmingJeffrey M Davidson
Oct 28, 2005·The Journal of Investigative Dermatology. Symposium Proceedings·Alfred S LewinLeonard M Milstone
Jan 11, 2008·The Journal of Investigative Dermatology·María J EscámezMarcela Del Río
May 15, 2008·The Journal of Investigative Dermatology·Jeffrey M Davidson
Dec 17, 2014·Developmental Cell·Marco DemariaJudith Campisi
May 22, 2007·Advanced Drug Delivery Reviews·Laura De Laporte, Lonnie D Shea
Feb 6, 2007·Clinics in Dermatology·Sabine A EmingJeffrey M Davidson
Apr 2, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·V ArulR Jayakumar
Feb 27, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tomonori NasuTakashi Nakamura
Oct 14, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Gerit MulderBarbara K Sosnowski
Oct 1, 2008·Journal of Periodontal Research·Z LinW V Giannobile
Jul 26, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Xiaoli WuWei Liu
May 12, 2006·Periodontology 2000·Jan Eirik EllingsenS Petter Lyngstadaas
Mar 5, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Peter BlumeBarbara K Sosnowski
Aug 4, 2001·The American Journal of Pathology·G F Pierce
Nov 28, 2006·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Russell R ReidThomas A Mustoe
Mar 7, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Guobao WeiPeter X Ma
Nov 3, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·M HaiderH Ghandehari
Jun 26, 2003·The Surgical Clinics of North America·Nicola C PetrieElof Eriksson
Nov 26, 2008·International Journal of Pharmaceutics·David HwangHamidreza Ghandehari

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