Matrix metalloprotease 16 expression is downregulated by microRNA-146a in spontaneously differentiating Caco-2 cells

Development, Growth & Differentiation
Erhan AstarciSreeparna Banerjee

Abstract

Cellular differentiation in the gut is vital in maintaining the cellular and functional specialization of the epithelial layer. MicroRNAs (miRNAs) have recently emerged as one of the key players in orchestrating the differentiation process in the gut. Using the spontaneously differentiating Caco-2 cell line, we observed an increased expression of miR-146a but not miR-146b in the course of differentiation. Bioinformatic analyses revealed that the membrane type matrix metalloprotease 16 (MMP16, MT3-MMP) was a predicted target of miR-146a and a decrease in the mRNA and protein expression of MMP16 was observed in the course of differentiation. Transfection of a luciferase reporter vector containing the 3'UTR of MMP16 showed decreased luciferase activity due to miR-146a expression. With forced expression of miR-146a in undifferentiated Caco-2 cells, a decrease in the mRNA and protein levels of MMP16 and a lower gelatinase activity in a gelatin zymogram were observed. Additionally, forced expression of miR-146a in HT-29 colon cancer cells also resulted in decreased expression of MMP16, along with a decrease in the invasion through Matrigel. Taken together, we have shown here that MMP16 is regulated by miR-146a in spontaneously differ...Continue Reading

References

Aug 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Konstantin D TaganovDavid Baltimore
Sep 20, 2006·Genes & Development·Kevin HotaryStephen J Weiss
Dec 16, 2006·Cell Biology and Toxicology·P Simon-AssmannM Kedinger
Sep 26, 2007·Nature Genetics·Michael KerteszEran Segal
Jan 5, 2008·RNA·Shi-Lung LinShao-Yao Ying
May 16, 2008·Nature Reviews. Cancer·Adam Humphries, Nicholas A Wright
Aug 21, 2008·Clinical Genetics·A E Erson, E M Petty
Oct 14, 2008·Trends in Cell Biology·R Grant Rowe, Stephen J Weiss
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Feb 7, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Cécile BeaurepaireDerek M McKay
Mar 6, 2009·Nature Reviews. Cancer·Kornelia Polyak, Robert A Weinberg
Mar 7, 2009·Brain Research·Hongping XiaMarie C Lin
Jul 20, 2010·Differentiation; Research in Biological Diversity·Guillaume DalmassoDidier Merlin
Jul 28, 2010·Gastroenterology·Lindsay B McKennaKlaus H Kaestner
Oct 19, 2010·Cell Host & Microbe·Cécilia ChassinMathias W Hornef
Jun 3, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ryunosuke KogoMasaki Mori

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Citations

Mar 29, 2014·Cancers·Mohammed AbbaHeike Allgayer
Jun 4, 2013·Journal of Cancer Research and Clinical Oncology·Meili LvLin Zhang
Apr 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Jennifer VandoorenGhislain Opdenakker
Aug 23, 2018·Journal of Cellular Biochemistry·Somaye NoruziHamed Mirzaei
Jul 28, 2017·Cellular Oncology (Dordrecht)·Georgios S MarkopoulosEvangelos Kolettas
Dec 17, 2020·Cancers·Tomasz M GrzywaPaweł K Włodarski

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