PMID: 11906929Mar 22, 2002Paper

Matrix metalloproteinase (2, 9, and 14) expression, localization, and activity in ovine corpora lutea throughout the estrous cycle

Biology of Reproduction
William A RickeMichael F Smith

Abstract

Members of the matrix metalloproteinase (MMP) family collectively degrade extracellular matrix (ECM) and help regulate luteal function. The objectives of these experiments were to characterize the mRNA expression, localization, and activity of MMPs 2, 9, and 14 in ovine corpora lutea (CL). Ovine CL were collected on Days 2, 4, 10, and 15 of the estrous cycle (Day 0 = estrus). Messenger RNA transcripts for MMPs 2 and 14 were detected using Northern analysis; however, expression of MMP-9 was undetectable. Expression of MMP-14 mRNA (membrane type-1 MMP) was increased (P < 0.05) on Day 4; whereas, expression of MMP-2 mRNA was highest (P < 0.05) on Day 10, which corresponded to the observed increases in gelatinolytic activity in luteal homogenates as measured by a fluroscein-labeled gelatin substrate assay. MMP 2 and 9 proteins were localized predominantly to large luteal cells (LLCs), whereas MMP-14 was localized primarily to cells other than LLCs as demonstrated by immunohistochemistry. Immunolocalization of MMP-2 to putative endothelial cells was also observed on Day 15. Localization of MMP activity was determined using in situ zymography. Luteal tissues contained gelatinolytic activity primarily localized pericellularly to vario...Continue Reading

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Citations

Jun 5, 2002·Molecular and Cellular Endocrinology·Michael F SmithGeorge W Smith
Feb 25, 2005·Biology of Reproduction·Ronit Haimov-KochmanSimcha Yagel
Nov 29, 2008·Biology of Reproduction·L C SchulzR M Roberts
Dec 24, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·M F SmithR Webb
May 3, 2014·Cell Biochemistry and Biophysics·Huayi WangFenglian Pan
Apr 1, 2006·Journal of Cellular Physiology·Chie-Hong WangWen-Chun Hung
Oct 29, 2008·Reproduction in Domestic Animals = Zuchthygiene·C EllenbergerW R Allen

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