Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles

Reproductive Biology and Endocrinology : RB&E
Wen-Jui YangChih-Sheng Lin

Abstract

To determine whether matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP-1 and TIMP-2) in human follicular fluid, have any relationships with oocyte maturation in vivo and subsequent fertilization during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles. The follicular fluids were obtained from 150 female patients undergoing IVF/ICSI cycles and a total of 1504 oocytes were retrieved for analysis. MMP-2 and MMP-9 activities were measured using zymography assay. TIMP-1 and TIMP-2 concentrations were quantitatively assessed using enzyme-linked immunosorbent assay (ELISA). Human follicular fluid MMP-2 level was significantly associated with the rate of maturity of oocytes (P < 0.001). Furthermore, the MMP-2 was significantly associated with the higher fertilization rate (P < 0.01). There was no significant correlation between follicular MMP-9 and the maturation rate of oocytes. The TIMP-1 and TIMP-2 also showed no correlation with the oocyte maturation rate. The level of gelatinase MMP-2 in human follicular fluid might be a reliable marker of mature oocytes during IVF/ICSI cycles. Furthermore, the MMP-2 expression has a strong association with higher fertilization rate. Further studies ar...Continue Reading

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Citations

Oct 28, 2016·Indian Journal of Microbiology·Sanjay KumarShreesh Ojha
Mar 3, 2018·Journal of Assisted Reproduction and Genetics·M G Da BroiP A Navarro
Nov 30, 2018·Endocrine Reviews·Diane M DuffyThomas E Curry
Mar 29, 2019·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Shirin SharifiHeidar Tavilani

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Methods Mentioned

BETA
zymography
ELISA

Software Mentioned

ImageJ
SPSS

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