Matrix metalloproteinase-9 activity detected in body fluids is the result of two different enzyme forms

Journal of Biochemistry
Tiziana BelliniMassimiliano Castellazzi

Abstract

In vitro activation of matrix metalloproteinase-9 (MMP-9) (Gelatinase B) with MMP-3 shows the presence of two different forms: an 82 kDa, N-terminal truncated form, and a 65 kDa, N- and C-terminal truncated form. So far the presence of the 65 kDa form has not been reported in vivo. Affinity chromatography was performed to separate MMP-9 from MMP-2 and immunoprecipitation to isolate ∼65 kDa MMP-9 from 82 kDa MMP-9 in sera of healthy donors. The presence of ∼65 kDa active MMP-9 was demonstrated both with gelatin zymography and western blot analysis. The ∼65 kDa MMP-9 lacks the haemopexin domain required for the high-affinity binding of the tissue inhibitor TIMP-1, and can be evaluated by activity assay in the presence of TIMP-1. This opens the possibility to investigate the role of this form of MMP-9 that escapes physiological regulation.

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Citations

Jun 6, 2013·Molecular and Cellular Biochemistry·Roberta RizzoTiziana Bellini
Jan 30, 2014·Cancers·Antonietta Rosella Farina, Andrew Reay Mackay
Jan 25, 2014·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Alessandro TrentiniCarlo A Volta
Jan 16, 2016·Psychoneuroendocrinology·Mateusz KucharczykBogusława Budziszewska
Mar 13, 2014·Journal of Cellular and Molecular Medicine·Rocco RossanoPaolo Riccio
Apr 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Jennifer VandoorenGhislain Opdenakker
Feb 11, 2015·Multiple Sclerosis : Clinical and Laboratory Research·Alessandro TrentiniUNKNOWN Emilia-Romagna network for Multiple Sclerosis (ERMES) study group
May 2, 2015·The European Respiratory Journal·Luke W GarrattUNKNOWN Australian Respiratory Early Surveillance Team for Cystic Fibrosis (AREST CF)
Nov 7, 2020·Matrix Biology : Journal of the International Society for Matrix Biology·Lise BoonGhislain Opdenakker
Mar 16, 2021·Matrix Biology Plus·Christoforos OdiatisConstantinos Deltas

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