Matrix metalloproteinase-9 and -7 are regulated in experimental autoimmune encephalomyelitis

Brain : a Journal of Neurology
B C KieseierH P Hartung

Abstract

Matrix metalloproteinases (MMPs) comprise a group of proteolytic enzymes that are implicated in the pathogenesis of inflammatory diseases of the nervous system such as multiple sclerosis. However, the exact function and expression pattern of MMPs in the inflamed nervous system are not known. In the present study we investigated the expression of 92-kDa gelatinase (MMP-9) in spinal cord from animals with adoptive transfer experimental autoimmune encephalomyelitis (AT-EAE), using a semiquantitative competitive reverse transcriptase-polymerase chain reaction assay. Increased levels of MMP-9 mRNA were found with peak values at times of maximum disease severity. Increased mRNA expression was associated with enhanced proteolytic activity of this enzyme, as demonstrated by gelatin zymography. Immunohistochemistry revealed immunoreactivity along the meninges, around blood vessels and within the parenchyma, in diseased but not in normal spinal cord. Furthermore, the expression pattern of five other MMPs was investigated. Matrilysin (MMP-7) was also found to be upregulated with maximum mRNA levels at the peak of the disease. In contrast, mRNAs for collagenase-3, 72-kDa gelatinase, and stromelysin-1 and -3 were not changed. Our findings i...Continue Reading

Citations

Jul 13, 2002·Developmental Psychobiology·Jonathan E Roberts, Martha Ann Bell
Nov 19, 2004·Journal of Neurology·Hans-Peter HartungYannis Zoukos
Aug 22, 2009·Cell and Tissue Research·Eva KorposLydia Sorokin
May 3, 2006·Journal of Clinical Immunology·Maya RamYehuda Shoenfeld
Sep 5, 2013·Acta neurologica Belgica·Mohammad A JavaidZeeshan Sheikh
Mar 7, 2003·Journal of Neuroimmunology·Carlo AvolioMaria Trojano
Jul 17, 2003·Journal of Neuroimmunology·Jorge Correale, María de los Milagros Bassani Molinas
Jan 18, 2003·Progress in Neurobiology·Margaret A Petty, Eng H Lo
Jul 20, 2002·Clinical Neurology and Neurosurgery·Ariel Miller, Yanina Galboiz
Jan 15, 2004·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Asha DasDuncan R Smith
Apr 10, 2002·Journal of the Peripheral Nervous System : JPNS·Veronica I Shubayev, Robert R Myers
Nov 13, 2004·Neuroreport·Thomas LadewigElke Guenther
Apr 11, 2006·Neuroreport·Fatima T HusainBarry Horwitz
May 3, 2000·International Ophthalmology Clinics·A D Dick
Jun 15, 2004·Neuroreport·Cesar A Arango-DávilaHernán J Pimienta
Apr 6, 2013·BMC Medicine·Neal KumarAlice B Gottlieb
Jan 8, 2009·PloS One·Wei HuOlaf Stüve
Jan 11, 2002·Reviews in the Neurosciences·M MikkonenR Miettinen
May 16, 2002·Reviews in the Neurosciences·Nuno Sousa, Osborne F X Almeida
Apr 1, 2014·Journal of Neuroinflammation·Mark StettnerBernd C Kieseier
Oct 7, 2014·Journal of Natural Products·Jing XuYuanqiang Guo
Sep 12, 2013·Clinical & Developmental Immunology·Helen Könnecke, Ingo Bechmann
Apr 16, 2013·Journal of Neurology, Neurosurgery, and Psychiatry·Kazuyuki SaitoTakashi Kanda
Apr 10, 2002·Acta Neurologica Scandinavica·B G H SchoserH-J Stuerenburg
Jul 14, 2010·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Jochen C UlzheimerHeinz Wiendl
Sep 28, 1999·Current Opinion in Neurology·B C KieseierH P Hartung
Sep 14, 2006·Current Opinion in Neurology·Bernd C KieseierHeinz Wiendl

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.