PMID: 11319136Apr 25, 2001Paper

Matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-3 are key regulators of extracellular matrix degradation by mouse embryos

Biology of Reproduction
E J WhitesideM B Harvey

Abstract

Embryo implantation in humans and rodents is a highly invasive yet tightly controlled process involving extracellular matrix (ECM) degradation. Matrix metalloproteinase 9 (MMP-9) has been implicated as the major facilitator of this ECM degradation. MMP-9 is expressed by the embryo's trophoblast cells, whereas tissue inhibitor of metalloproteinases 3 (TIMP-3) is expressed by the maternal uterine cells immediately adjacent to the trophoblast. We examined the functional roles of MMP-9 and TIMP-3 during in vitro ECM degradation by mouse embryos. Blastocysts were treated with either MMP-9 antisense or sense oligonucleotides and incubated on an ECM gel. The extent of ECM degradation exhibited by the blastocysts due to proteinase secretion was quantified. Embryos exposed to MMP-9 antisense oligonucleotides exhibited reduced ECM-degrading activity as compared with controls, and this reduced activity was correlated with the level of MMP-9 secreted by the embryos. The functional role of TIMP-3 was then examined by incubating blastocysts on an ECM gel that had been impregnated with various amounts of TIMP-3. In a dose-dependent manner, increases in TIMP-3 resulted in a reduction in ECM degradation and were correlated with diminished MMP-9...Continue Reading

References

Oct 1, 1992·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C H Graham, P K Lala
Jan 20, 1992·FEBS Letters·W G Stetler-StevensonD W Golde
Oct 30, 1992·Cell·S Strickland, W G Richards
Apr 1, 1991·The Journal of Cell Biology·C L LibrachS J Fisher
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G I GoldbergC S He
Apr 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P ReponenK Tryggvason
Jan 1, 1995·Methods in Enzymology·G Murphy, T Crabbe
Jul 1, 1993·Molecular Reproduction and Development·P WaterhouseR Khokha
Jun 25, 1993·Nucleic Acids Research·I M Khan, J M Coulson
Sep 11, 1993·Nucleic Acids Research·I M Khan, J M Coulson
Jun 1, 1996·Seminars in Cancer Biology·W G Stetler-StevensonM Corcoran
Oct 2, 1998·FEBS Letters·A AmourG Murphy

❮ Previous
Next ❯

Citations

Mar 30, 2006·Reproductive Biomedicine Online·Carolyn B CoulamRoumen Roussev
Nov 16, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·M G Martínez-HernándezD Randall Armant
Feb 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Li ChenRomana A Nowak
Jun 9, 2012·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Chih-Liang TienMingli Hsieh
Aug 21, 2009·Journal of Cellular Biochemistry·Jen-Hsiang TsaiShao-Hsuan Kao
Dec 13, 2006·American Journal of Reproductive Immunology : AJRI·Jana SkrzypczakMateusz Mikolajczyk
Jan 27, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·David K Gardner
Aug 29, 2006·Biochemical and Biophysical Research Communications·Claire E QuinnThomas G Kennedy
Feb 2, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Cleofina BoscoGernika García
Nov 1, 2005·The Journal of Thoracic and Cardiovascular Surgery·Paul W M FedakRen-Ke Li
Apr 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Jennifer VandoorenGhislain Opdenakker
Mar 26, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Valerie ArpinoSean E Gill
Feb 3, 2018·Molecular Reproduction and Development·Alejandra Sánchez-SantosLuis A Baiza-Gutman
Apr 13, 2018·Expert Review of Proteomics·Mustapha BenkhalifaMonia Allal-Elasmi
Dec 26, 2001·FEBS Letters·P CostaglioliB Garbay
May 28, 2005·Human Reproduction·Huei-Wen ChenChii-Ruey Tzeng
Jan 9, 2017·Biological Trace Element Research·Hong-Wei WangBian-Hua Zhou
Mar 20, 2019·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Claudia Castruita-De la RosaMargarita L Martinez-Fierro
Jan 10, 2021·International Journal of Molecular Sciences·Griselda A Cabral-PachecoMargarita L Martinez-Fierro
Oct 15, 2020·Journal of Receptor and Signal Transduction Research·Ying CuiQiubo Lv

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Related Papers

Microcirculation : the Official Journal of the Microcirculatory Society, Inc
Donald G Welsh, Mark S Taylor
Combinatorial Chemistry & High Throughput Screening
Zheng QiuGhislain Opdenakker
© 2021 Meta ULC. All rights reserved