Matrix metalloproteinase-9 in a unique proteoglycan form in avian embryonic growth plate cartilage.

Archives of Biochemistry and Biophysics
R Krishna R PatchigollaT M Schmid

Abstract

The appearance of a high molecular weight gelatinolytic enzyme (230 kDa) correlated with cartilage collagen loss in chick embryonic tibias cultured with lipopolysaccharide. This 230 kDa enzyme was purified and its activity was measured on synthetic and natural substrates. The enzyme was activated by aminophenylmercuric acetate and inhibited by ethylenediaminetetraacetic acid, phenanthroline, marimastat or tissue inhibitors of metalloproteinases. Amino acid sequences of peptides derived from the purified enzyme showed identity with avian MMP-9. Digestion of the intact enzyme with chondroitinase decreased the size of the molecule to 80 kDa on SDS-PAGE. When chick embryonic tibia cultures were radiolabeled with (35)S-sulfate, the radiolabel co-purified with the 230 kDa gelatinase. Chondroitinase treated 230 kDa gelatinase also reacted with specific anti-chondroitin sulfate antibodies and FACE analysis revealed a predominance of chondroitin-4-sulfate. These results demonstrate this avian matrix metalloproteinase contained glycosaminoglycan chains. To our knowledge, this is the first report of a matrix metalloproteinase in a proteoglycan form.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Mar 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A A ColeT M Schmid
Jul 8, 1991·Biochimica Et Biophysica Acta·F M CraigG Murphy
Dec 1, 1987·Archives of Biochemistry and Biophysics·Y Mikuni-Takagaki, Y S Cheng
Oct 1, 1989·Current Opinion in Cell Biology·C M Alexander, Z Werb
Aug 1, 1985·The Journal of Clinical Investigation·D D DeanD S Howell
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M A BourdonE Ruoslahti
Dec 1, 1985·The Journal of Experimental Medicine·A J SantB D Schwartz
Feb 1, 1985·Developmental Biology·T M Schmid, T F Linsenmayer
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Aug 31, 1982·Biochemical and Biophysical Research Communications·H Birkedal-Hansen, R E Taylor
Sep 6, 1994·Annals of the New York Academy of Sciences·J F Woessner
May 15, 1994·Archives of Biochemistry and Biophysics·B L SchumacherK E Kuettner
Oct 11, 1996·The Journal of Biological Chemistry·M YangM Kurkinen
Jul 1, 1997·Research in Veterinary Science·M W OrthT M Schmid
Jun 17, 2000·Arthritis and Rheumatism·Y NishidaW Knudson
Aug 14, 2001·Biochemical and Biophysical Research Communications·B E PfisterT M Schmid
Nov 1, 2001·Biochimica Et Biophysica Acta·P E Van den SteenP M Rudd
Jun 20, 2003·Annals of the New York Academy of Sciences·Nathalie OrtegaZena Werb
Aug 22, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A TongE Monsonego-Ornan
Dec 22, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Charles J Malemud
Jan 13, 2006·Cardiovascular Research·Hideaki NagaseGillian Murphy
May 5, 2006·The Journal of Biological Chemistry·Philippe E Van den SteenGhislain Opdenakker
May 20, 2008·Bone·Stephen M Krane, Masaki Inada
Aug 2, 2008·Archives of Biochemistry and Biophysics·Anne-Marie MalfaitMicky D Tortorella
Jul 21, 2009·Current Opinion in Cell Biology·Charlotte J MorrisonChristopher M Overall
Sep 4, 2010·Critical Reviews in Biochemistry and Molecular Biology·Bénédicte Cauwe, Ghislain Opdenakker
Feb 22, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jun LiAnna Plaas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Related Papers

Annals of the New York Academy of Sciences
A A ColeT M Schmid
Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
N C RathH Xie
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
D JosićW Reutter
© 2022 Meta ULC. All rights reserved