Matrix metalloproteinase expression is altered in the small and large intestine following fractionated radiation in vivo

Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer
Romany StansboroughRachel Gibson

Abstract

Radiotherapy-induced gut toxicity (RIGT) is associated with significant diarrhoea, pain and rectal bleeding. Matrix metalloproteinases (MMPs) have been reported to be involved in chemotherapy-induced gut toxicity and RIGT following single-dose irradiation in vivo. We therefore proposed MMPs would be involved in the pathobiology of RIGT following fractionated irradiation. Dark Agouti rats were treated with fractionated radiation (3 × 2.5 Gy/week for 6 weeks). Rats were killed at 3, 6 and 15 weeks to represent acute and chronic toxicities. Sections of jejunum and colon were immunostained for MMP-1, MMP-2, MMP-9 and MMP-14. Relative mRNA expression in jejunum and colon was quantified by RT-PCR for MMP-1, MMP-2, MMP-9 and MMP-14. Western blotting was also conducted on jejunum and colon tissue collected at week 6 to determine protein levels of pro- and active MMP-2. MMP-2 total protein levels, determined by western blotting, significantly increased in both the jejunum (p = 0.0359) and the colon (p = 0.0134) 6 weeks into the fractionated radiation schedule. MMP-1, MMP-2, and MMP-14 mRNA expression significantly increased in the jejunum. MMP-2 mRNA expression was also significantly increased in the colon. Immunostaining of MMP-2 was o...Continue Reading

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Citations

Jul 25, 2019·Current Opinion in Clinical Nutrition and Metabolic Care·Michael Thomsen, Luis Vitetta
Oct 1, 2019·Journal of Cellular and Molecular Medicine·María Auxiliadora Olivares-UrbanoMaría Isabel Núñez
Mar 30, 2019·Current Opinion in Supportive and Palliative Care·Hannah R WardillAndrea M Stringer
Jun 1, 2021·Frontiers in Oncology·Verena Waller, Martin Pruschy
Jul 3, 2021·Nature Reviews. Immunology·Urszula M CytlakTimothy M Illidge

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