Matrix metalloproteinase (MMP)-7 in Barrett's esophagus and esophageal adenocarcinoma: expression, metabolism, and functional significance.

Physiological Reports
Hanan M GarallaAndrea Varro

Abstract

Matrix metalloproteinase (MMP)-7, unlike many MMPs, is typically expressed in epithelial cells. It has been linked to epithelial responses to infection, injury, and tissue remodeling including the progression of a number of cancers. We have now examined how MMP-7 expression changes in the progression to esophageal adenocarcinoma (EAC), and have studied mechanisms regulating its expression and its functional significance. Immunohistochemistry revealed that MMP-7 was weakly expressed in normal squamous epithelium adjacent to EAC but was abundant in epithelial cells in both preneoplastic lesions of Barrett's esophagus and EAC particularly at the invasive front. In the stroma, putative myofibroblasts expressing MMP-7 were abundant at the invasive front but were scarce or absent in adjacent tissue. Western blot and ELISA revealed high constitutive secretion of proMMP-7 in an EAC cell line (OE33) that was inhibited by the phosphatidylinositol (PI) 3-kinase inhibitor LY294002 but not by inhibitors of protein kinase C, or MAP kinase activation. There was detectable proMMP-7 in cultured esophageal myofibroblasts but it was undetectable in media. Possible metabolism of MMP-7 by myofibroblasts studied by proteomic analysis indicated degra...Continue Reading

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Citations

Feb 23, 2020·Cells·Antonio PalumboLuiz Eurico Nasciutti
Aug 9, 2020·Cancer Management and Research·Peng HanFan Li
Nov 1, 2020·International Journal of Molecular Sciences·Fran QuiltyCarlos Medina
Mar 14, 2021·Clinical Biochemistry·Hai-Yang LiaoHai-Hong Zhang
Dec 7, 2021·Cancer Medicine·Ksenia S MaslyonkinaLiudmila M Mikhaleva

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Methods Mentioned

BETA
biopsies
ELISA
electrophoresis
PMA

Software Mentioned

Mascot

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