Matrix metalloproteinases and their inhibitors in human cumulus and granulosa cells as biomarkers for oocyte quality estimation

Fertility and Sterility
Alice LuddiVincenzo de Leo

Abstract

To study the molecular profile of metalloproteinases and their tissue inhibitors in granulosa and cumulus cells in a subset of fertile and infertile women. Molecular study with granulosa and cumulus cells. University hospital. Forty-four women undergoing assisted reproductive techniques for female infertility factor, with partners having a normal spermiogram and 15 normally fertile women with male partner affected by severe oligoasthenoteratozoospermia or nonobstructive azoospermia. In vitro fertilization. We investigated gene expression level of metalloproteinases (MMP2, MMP9, MMP11) and their tissue inhibitors (TIMP1, TIMP2) by means of quantitative reverse-transcription polymerase chain reaction, protein quantification by means of Western blot, and localization by means of immunofluorescence. We firstly validated HPRT1 as the most reliable housekeeping gene enabling correct gene expression analysis in both granulosa and cumulus cells. Gene expression, Western blot, and immunofluorescence analysis of MMP2, MMP9, and MMP11 and their tissue inhibitors TIMP1 and TIMP2 demonstrated that these enzymes are finely tuned in these cells. MMP9 is specifically expressed only in granulosa, whereas MMP2 is more expressed in cumulus and gr...Continue Reading

Citations

Apr 25, 2020·International Journal of Molecular Sciences·Alice LuddiPaola Piomboni
Mar 20, 2021·Reproductive Biomedicine Online·Caroline M Da LuzPaula A Navarro
Jun 22, 2021·Cell and Tissue Banking·Francisco Denilson Rodrigues GomesAna Paula Ribeiro Rodrigues

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