Matrix metalloproteinases and their tissue inhibitors in the developing neonatal mouse uterus

Biology of Reproduction
Jianbo HuThomas E Spencer

Abstract

Postnatal development of the mouse uterus involves differentiation and development of the endometrial glands as well as the myometrium. Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are involved in extracellular matrix breakdown and morphogenesis of many epitheliomesenchymal organs. As a first step to understanding their roles in postnatal mouse uterine development, MMPs and TIMPs found to be expressed in the neonatal mouse uterus by microarray analysis were localized by in situ hybridization. The MMP-2 mRNA was detected only in the uterine stroma, whereas the MMP-10 mRNA was present only in the uterine epithelium from Postnatal Day (PND) 3 to PND 9. All other MMPs (MMP-11, MMP-14, and MMP-23) as well as TIMP-1, TIMP-2, and TIMP-3 were detected in both epithelial and stromal cells of the endometrium, but not in the myometrium. Uterine extracts were then analyzed by gelatin and casein gel zymography to detect active gelatinases and stromelysins, respectively. Five major gelatinase bands of activity were detected and inhibited by the MMP inhibitors, EDTA or 1,10-phenanthroline, but not by PMSF, a serine protease inhibitor. Western blot analysis confirmed the presence of MMP-2 and MMP-9 proteins in the uteru...Continue Reading

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Feb 27, 2004·Biology of Reproduction·Jianbo HuThomas E Spencer

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Citations

Mar 25, 2005·Biology of Reproduction·Jianbo Hu, Thomas E Spencer
Jan 13, 2012·Biology of Reproduction·Justyna FilantThomas E Spencer
Dec 25, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Joseph C ChenCarol A Bagnell
Feb 15, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Joseph C ChenCarol A Bagnell
Apr 10, 2009·Kidney International·Angel A Soler-GarcíaPatricio E Ray
Sep 1, 2006·Biochemical and Biophysical Research Communications·Dan HuTatsuya Yamagata
Oct 20, 2011·Molecular and Cellular Endocrinology·Thomas E SpencerJustyna Filant
Feb 26, 2013·Journal of Reproductive Immunology·Myriam Hemberger
Sep 24, 2014·Reproductive Toxicology·Romina HigaAlicia Jawerbaum
Jan 18, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Ashley F GeorgeCarol A Bagnell
Jul 23, 2009·Physiological Genomics·Nadéra Mansouri-AttiaOlivier Sandra
May 16, 2020·Balsaeng'gwa saengsig·Yong-Pil Cheon
Jul 9, 2005·Journal of Biochemistry·Yan Yin, Liang Ma

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Methods Mentioned

BETA
PCR
reverse transcription-PCR
in vitro transcription
Protein Assay
electrophoresis
zymography

Software Mentioned

Biosystems
Primer Express
Applied
Statistical Analysis System
BLAST

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