Matrix with high salt tolerance for the analysis of peptide and protein samples by desorption/ionization time-of-flight mass spectrometry

Analytical Chemistry
Songyun XuHanfa Zou

Abstract

High concentrations of urea and guanidine hydrochloride are commonly used for the denaturation of protein, which was digested by enzymatic proteolysis for the identification by MS analysis. The presence of these contaminants seriously suppresses the ion signal of analytes in MALDI-TOF MS analysis. Herein, a novel MALDI matrix, 3, 4-diaminobenzophenone (DABP), has been found with high tolerance for these contaminants in MALDI MS analysis. The ion signal of analyte insulin can be detected in the presence of 2 M guanidine hydrochloride and 1.5 M urea using DABP as matrix. The tryptic digest of BSA (400 fmol) in 1 M guanidine hydrochloride or 1 M urea was successfully analyzed without any pretreatment prior to MS analysis. Furthermore, it has been found that this matrix can also effectively suppress the cation ion adduction of the peptides in the presence of high concentrations of metal ions in sample solution.

References

Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J HenzelC Watanabe
Jan 1, 1997·Trends in Biochemical Sciences·T Hunter, G D Plowman
Oct 8, 1999·Rapid Communications in Mass Spectrometry : RCM·V E Vandell, P A Limbach
Mar 25, 2000·Science·M D AdamsJ C Venter
Feb 9, 2002·Mass Spectrometry Reviews·C Fenselau, P A Demirev
Aug 8, 2002·Proteomics·Simon EkströmGyörgy Marko-Varga
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·O Vorm, M Mann

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Citations

Nov 7, 2006·Analytical and Bioanalytical Chemistry·Chensong PanHanfa Zou
Nov 1, 2007·Chemical Communications : Chem Comm·Ying ZhangPengyuan Yang
May 8, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao-Ting LiuYi-Min Zhao
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Nov 16, 2018·Rapid Communications in Mass Spectrometry : RCM·Gongyu LiLingjun Li
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·Yu FuHanfa Zou
Jul 23, 2008·Journal of Proteome Research·Michael Schilling, Daniel R Knapp
Feb 2, 2010·Analytica Chimica Acta·Guoqiang ChenZhili Li

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