Maturation and degradation of beta-galactosidase in the post-Golgi compartment are regulated by cathepsin B and a non-cysteine protease

FEBS Letters
Y Okamura-OhoY Suzuki

Abstract

Lysosomal beta-galactosidase precursor is processed to a mature form and associated with protective protein in lysosomes. In this study we used two cysteine protease proinhibitors, E64-d for cathepsins B, S, H, and L, and CA074Me for cathepsin B. They are converted intracellularly to active forms, E-64c and CA074, respectively. Both active compounds inhibited maturation of the exogenous beta-galactosidase precursor, but E-64c did not inhibit further degradation to an inactive 50-kDa product. We concluded that cathepsin B participated exclusively in maturation of beta-galactosidase, and a non-cysteine protease was involved in further degradation and inactivation of the enzyme molecule.

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Citations

Mar 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Izabela Berdowska
Sep 18, 2008·Protein Science : a Publication of the Protein Society·Patricia SchenkerAntonio Baici
Mar 16, 2002·Oncogene·Christopher J Howlett, Stephen M Robbins
Jun 1, 2013·Biomolecular Concepts·Marko Novinec, Brigita Lenarčič

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