PMID: 9557943Apr 29, 1998Paper

Maturation-dependent gene expression in a conditionally transformed liver progenitor cell line

In Vitro Cellular & Developmental Biology. Animal
A S FiorinoL M Reid

Abstract

We have isolated a conditionally transformed liver progenitor cell line with phenotypic similarities to both hepatoblasts (bipotent embryonic liver cells that give rise to hepatocytes and intrahepatic biliary epithelial cells) and liver epithelial cells (primitive hepatic cells isolated from adult livers capable of generating both hepatocytic and biliary lineages). Cell line L2039 was derived from E14 fetal mouse liver after transformation with temperature-sensitive SV-40 large T antigen. At 33 degrees C, these cells have an epithelial morphology with a high nucleocytoplasmic ratio and express both hepatocytic and biliary genes, including albumin, alpha-fetoprotein, glutamine synthetase, insulinlike growth factor II receptor, fibronectin and laminin, and cytokeratins 8 and 19, a set of markers characteristic for hepatoblasts. The presence of cytokeratin 14, vimentin, and several oval-cell antigens link cell line L2039 to nonparenchymal liver epithelial cell populations thought to contain progenitor cells. Serum-free, hormonally defined media conditions and extracellular matrix requirements were determined for growth and differentiation of this cell line. During culture on type IV collagen at 39 degrees C, L2039 cells cease divi...Continue Reading

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Citations

Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T MitakaY Mochizuki
Oct 20, 2010·Cell and Tissue Research·Caroline Beth Sangan, David Tosh
Jul 3, 2007·Journal of Molecular Histology·Elke UeberhamRolf Gebhardt
Dec 7, 2007·Journal of Cellular and Molecular Medicine·Elke UeberhamUwe Ueberham
Sep 26, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hélène Strick-Marchand, Mary C Weiss
May 12, 2016·Asian-Australasian Journal of Animal Sciences·Yunsheng ZhangShuisheng Hou

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