Maturation of pig oocytes: observations on membrane potential

Biology of Reproduction
M MattioliE Seren

Abstract

The membrane-potential changes of pig oocytes during maturation are described. Cumulus-enclosed oocytes have a resting potential of -41.81 +/- 0.60 mV; the removal of cumulus cells caused this potential to drop to -30.95 +/- 0.43 mV. Adding LH to the culture medium did not influence the potential of denuded oocytes but depolarized the potential of cumulus-enclosed oocytes to -32.90 +/- 0.43 mV. FSH did not affect the membrane potential of denuded or cumulus-enclosed oocytes, but significantly reduced the amplitude of the depolarization induced by LH. The effect of gonadotropins on cultured granulosa cells was also investigated. Plated granulosa cells have a resting potential of -45.21 +/- 0.72 mV, similar to that of cumulus-enclosed oocytes. As recorded in cumulus-enclosed oocytes, LH depolarized granulosa cell membrane potential (-30.33 +/- 0.69 mV) and FSH reduced this effect. To evaluate if oocyte maturation in vivo is accompanied by membrane-potential depolarization, follicular growth and oocyte maturation were induced in 6 prepubertal gilts by using an eCG-hCG treatment. Twenty hours after the beginning of oocyte maturation in vivo (induced by hCG), the membrane potential of the oocyte was depolarized to -28.84 +/- 1.01 mV...Continue Reading

Citations

Oct 1, 1994·Pflügers Archiv : European journal of physiology·G MealingJ L Schwartz
Oct 26, 2005·Veterinary Research Communications·L GioiaM Mattioli
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Nov 1, 1994·Zygote : the Biology of Gametes and Early Embryos·M Mattioli
Sep 18, 2010·Human Reproduction·Qingyou DuAleksandar Jovanović
Jun 18, 2003·Reproductive Biology and Endocrinology : RB&E·Pia LucidiMauro Mattioli
Jun 10, 2011·Molecular Reproduction and Development·F SilvestreE Tosti
Nov 1, 1976·Seminars in Arthritis and Rheumatism·F Fernandez-Madrid, M Mattioli
Dec 31, 1990·Biochemical and Biophysical Research Communications·B RubinskyA L Devries
Nov 30, 2018·Biology of Reproduction·Daniel Bahena-AlvarezArturo Hernández-Cruz
Jul 1, 2006·Expert Review of Endocrinology & Metabolism·Artur Mayerhofer, Lars Kunz
Dec 26, 2001·Molecular Pharmacology·Diane E MasonLisa C Freeman

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