PMID: 9529086Apr 7, 1998Paper

Maturation of the arginine-specific proteases of Porphyromonas gingivalis W50 is dependent on a functional prR2 protease gene

Infection and Immunity
Joseph Aduse-OpokuM A Curtis

Abstract

The prpR1 of Porphyromonas gingivalis codes for three distinct enzymes with specificity for arginyl peptide bonds termed RI, RIA, and RIB. These three isoforms comprise the majority of the extracellular, arginine-specific protease activity in P. gingivalis W50. RI is a heterodimer in which the catalytic alpha chain is noncovalently associated with a second chain involved in adherence phenomena. RIA and RIB are both monomeric species. RIA represents the free alpha chain, and RIB is a highly posttranslationally modified form of the alpha chain which is exclusively vesicle or membrane associated and migrates as a diffuse band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In previous studies, insertional inactivation of the prpR1 demonstrated that arginine-specific protease activity can also arise from a closely related second gene, prR2. In the present work, the prR2 was insertionally inactivated in P. gingivalis W50 in order to establish the contribution of this locus to the arginine-specific protease activity of this periodontal bacterium. Loss of prR2 function had several effects on prpR1-derived enzymes. First, the total Arg-X activity was reduced by approximately 50% relative to that of the parent strain. The ...Continue Reading

References

Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Apr 1, 1995·Infection and Immunity·H M FletcherF L Macrina
Nov 1, 1995·Trends in Microbiology·J PotempaJ Travis

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Citations

Nov 10, 1998·Oral Microbiology and Immunology·H K Kuramitsu
Oct 16, 1999·Periodontology 2000·S C HoltC A Genco
Feb 26, 2005·Infection and Immunity·Elaine VanterpoolHansel M Fletcher
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Hanne L P Tytgat, Sarah Lebeer
Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A CurtisM Rangarajan
Oct 24, 1998·Infection and Immunity·M TokudaH K Kuramitsu

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