PMID: 4557772Aug 1, 1972

Maturation of the immkune response in vitro. Focal fluctuation and changes in affinity of anti-beta-D-galactosidase activating antibody

The Journal of Experimental Medicine
A J MacarioF Celada

Abstract

We have cultivated lymph node microfragments from beta-D-galactosidase (Escherichia coli) primed rabbits and have measured their secondary response directed towards the whole molecule (precipitating antibodies) and to a single determinant (activating antibodies) of the antigen. By decreasing the size of the fragments to 10(5) cells, we began to observe heterogeneity among identical cultures in terms of positivity of response, antibody specificity, and titers. The affinity of "early" activating antibodies was inversely proportional to the dose of challenge. While no maturation was seen in low and excessive challenge, in all cultures receiving intermediate doses the association constant was raised several orders of magnitude within periods of 20 days. The relevance of these data to the mechanism of affinity selection of antigen-sensitive cells is discussed.

References

Dec 1, 1967·The Journal of Experimental Medicine·L A Steiner, H N Eisen
Dec 1, 1967·The Journal of Experimental Medicine·L A Steiner, H N Eisen
Jun 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·M B Rotman, F Celada
Jun 1, 1963·The Journal of Experimental Medicine·M C MICHAELIDES, A H COONS
Jul 1, 1964·Biochemistry·H N Eisen, G W SISKIND

Citations

Sep 1, 1974·Immunochemistry·A J Macario, E Conway de Macario
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J E AubinV Ling
Jan 1, 1974·Scandinavian Journal of Immunology·A J Cunningham, L M Pilarski
Mar 1, 1979·European Journal of Immunology·E Conway de MacarioG Biozzi
Oct 5, 2012·Scandinavian Journal of Immunology·I Lefkovits

Related Concepts

Antibody Formation
Antigen-Antibody Reactions
Alkalescens-Dispar Group
Galactosidase
Lymph Nodes
Precipitin Tests

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.