PMID: 4557772Aug 1, 1972

Maturation of the immkune response in vitro. Focal fluctuation and changes in affinity of anti-beta-D-galactosidase activating antibody

The Journal of Experimental Medicine
A J MacarioF Celada


We have cultivated lymph node microfragments from beta-D-galactosidase (Escherichia coli) primed rabbits and have measured their secondary response directed towards the whole molecule (precipitating antibodies) and to a single determinant (activating antibodies) of the antigen. By decreasing the size of the fragments to 10(5) cells, we began to observe heterogeneity among identical cultures in terms of positivity of response, antibody specificity, and titers. The affinity of "early" activating antibodies was inversely proportional to the dose of challenge. While no maturation was seen in low and excessive challenge, in all cultures receiving intermediate doses the association constant was raised several orders of magnitude within periods of 20 days. The relevance of these data to the mechanism of affinity selection of antigen-sensitive cells is discussed.


Dec 1, 1967·The Journal of Experimental Medicine·L A Steiner, H N Eisen
Dec 1, 1967·The Journal of Experimental Medicine·L A Steiner, H N Eisen
Jun 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·M B Rotman, F Celada
Jun 1, 1963·The Journal of Experimental Medicine·M C MICHAELIDES, A H COONS
Jul 1, 1964·Biochemistry·H N Eisen, G W SISKIND


Sep 1, 1974·Immunochemistry·A J Macario, E Conway de Macario
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J E AubinV Ling
Jan 1, 1974·Scandinavian Journal of Immunology·A J Cunningham, L M Pilarski
Mar 1, 1979·European Journal of Immunology·E Conway de MacarioG Biozzi
Oct 5, 2012·Scandinavian Journal of Immunology·I Lefkovits

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Antibody Formation
Antigen-Antibody Reactions
Alkalescens-Dispar Group
Lymph Nodes
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