PMID: 8446600Mar 1, 1993Paper

Maturation of thylakoid lumen proteins proceeds post-translationally through an intermediate in vivo

Proceedings of the National Academy of Sciences of the United States of America
G Howe, S Merchant

Abstract

Many thylakoid lumen proteins are synthesized outside the chloroplast as larger molecular weight precursors and then processed to their mature size during transport to the lumenal space. We have examined the post-translational processing of thylakoid lumen proteins in vivo by pulse-radiolabeling experiments with Chlamydomonas reinhardtii. Antibodies against the lumenal protein cytochrome c6 specifically immunoprecipitated three polypeptides from extracts of briefly pulse-radiolabeled cells. The molecular weights and kinetics of synthesis and turnover indicate that these three polypeptides are (i) the full-length cytochrome c6 precursor, (ii) a partially processed precursor (intermediate), and (iii) the completely processed mature protein. Identification of analogous forms of two other lumenal proteins, plastocyanin and the oxygen evolving enhancer 1 protein, indicates that the maturation of thylakoid lumen proteins occurs post-translationally in vivo and that the partially processed intermediate is a general feature of the pathway. The intermediate form of cytochrome c6 accumulated to a greater extent in cells incubated at 10 degrees C, relative to cells incubated at 22 degrees C, concomitantly with a decrease in the accumulati...Continue Reading

Citations

May 1, 1994·Photosynthesis Research·G Howe, S Merchant
Oct 1, 1994·Plant Molecular Biology·C Robinson, R B Klösgen
Nov 11, 2003·Trends in Plant Science·Martin WeigelDario Leister
Aug 7, 1998·Biochimica Et Biophysica Acta·Z Xie, S Merchant
Mar 14, 2000·Current Opinion in Plant Biology·A R Grossman
Jul 16, 2010·The Journal of Biological Chemistry·Stéphane T GabillyPatrice P Hamel
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Danny J. Schnell
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Cline, R Henry
Aug 28, 1998·Molecular Microbiology·R KranzS Merchant
Apr 1, 1994·Molecular Microbiology·L Thöny-MeyerH Hennecke
Aug 12, 2017·Frontiers in Plant Science·Stéphane T Gabilly, Patrice P Hamel
Dec 6, 1996·The Journal of Biological Chemistry·H H LiS Merchant
Mar 1, 1996·The Journal of Biological Chemistry·Z Xie, S Merchant
Aug 18, 2000·Biochimie·S S NakamotoS Merchant

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