Maturational Changes in Mouse Cutaneous Touch and Piezo2-Mediated Mechanotransduction.

Cell Reports
Niklas MichelManuela Schmidt

Abstract

The age of studied animals has a profound impact on experimental outcomes in animal-based research. In mice, age influences molecular, morphological, physiological, and behavioral parameters, particularly during rapid postnatal growth and maturation until adulthood (at 12 weeks of age). Despite this knowledge, most biomedical studies use a wide-spanning age range from 4 to 12 weeks, raising concerns about reproducibility and potential masking of relevant age differences. Here, using mouse behavior and electrophysiology in cultured dorsal root ganglia (DRG), we reveal a decline in behavioral cutaneous touch sensitivity and Piezo2-mediated mechanotransduction in vitro during mouse maturation but not thereafter. In addition, we identify distinct transcript changes in individual Piezo2-expressing mechanosensitive DRG neurons by combining electrophysiology with single-cell RNA sequencing (patch-seq). Taken together, our study emphasizes the need for accurate age matching and uncovers hitherto unknown maturational plasticity in cutaneous touch at the level of behavior, mechanotransduction, and transcripts.

References

Sep 11, 1992·Pflügers Archiv : European journal of physiology·D W McBride, O P Hamill
Jan 1, 1992·Journal of Gerontology·J C Stevens
Sep 1, 1988·Mechanisms of Ageing and Development·B ScottB Cinader
Nov 1, 1985·Developmental Psychobiology·I Golani, J C Fentress
Oct 17, 1998·Journal of Pharmacokinetics and Biopharmaceutics·R C Gagnon, J J Peterson
Oct 9, 1999·Neuroscience Letters·G C McCarterJ D Levine
Mar 29, 2001·Physiological Reviews·O P Hamill, B Martinac
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Apr 12, 2002·Nature·Elizabeth J BradburyStephen B McMahon
Nov 26, 2009·Ageing Research Reviews·Miaozong WuEric R Blough
Dec 2, 2009·The Journal of Physiology·François RugieroJohn N Wood
Jul 16, 2011·Annual Review of Cell and Developmental Biology·Nancy J LinfordScott D Pletcher
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Mar 19, 2013·BMC Bioinformatics·Charlotte Soneson, Mauro Delorenzi
Apr 12, 2013·Nature Communications·N EijkelkampJ N Wood
Oct 17, 2013·Toxicologic Pathology·Noel Downes, Pamela Mullins
Mar 26, 2014·Nature Communications·Kate PooleGary R Lewin
Apr 11, 2014·Nature·Seung-Hyun WooArdem Patapoutian
Oct 31, 2014·Nature Communications·Jeremy Tsung-chieh ChenJing Hu
Oct 31, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Luca AvenaliManuela Schmidt
Nov 25, 2014·Nature Neuroscience·Dmitry UsoskinPatrik Ernfors
Dec 5, 2014·Nature·Sanjeev S RanadeArdem Patapoutian
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jan 1, 2011·Current Protocols in Mouse Biology·Michael S MinettJohn N Wood
Jul 15, 2015·Molecular Reproduction and Development·Johannes SchindelinKevin W Eliceiri
Jul 30, 2015·Experimental Neurology·Maria Fitzgerald, Rebecca McKelvey
Dec 9, 2015·Annual Review of Biomedical Engineering·Jude M PhillipDenis Wirtz
Dec 17, 2015·Molecular Biology of the Cell·Race DiLoreto, Coleen T Murphy
Jan 28, 2016·Frontiers in Zoology·Vera BrustLars Lewejohann
Mar 2, 2016·The Journal of Biological Chemistry·Zhanfeng JiaJianguo G Gu
Mar 5, 2016·ELife·Oscar Flórez-VargasGoran Nenadic

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