Maturational differences in lung NF-kappaB activation and their role in tolerance to hyperoxia

The Journal of Clinical Investigation
Guang YangPhyllis A Dennery

Abstract

Neonatal rodents are more tolerant to hyperoxia than adults. We determined whether maturational differences in lung NF-kappaB activation could account for the differences. After hyperoxic exposure (O2 > 95%), neonatal (<12 hours old) lung NF-kappaB binding was increased and reached a maximum between 8 and 16 hours, whereas in adults no changes were observed. Additionally, neonatal NF-kappaB/luciferase transgenic mice (incorporating 2 NF-kappaB consensus sequences driving luciferase gene expression) demonstrated enhanced in vivo NF-kappaB activation after hyperoxia in real time. In the lungs of neonates, there was a propensity toward NF-kappaB activation as evidenced by increased lung I-kappaB kinase protein levels, I-kappaBalpha phosphorylation, beta-transducin repeat-containing protein levels, and total I-kappaBalpha degradation. Increased lung p-JNK immunoreactive protein was observed only in the adult lung. Inhibition of pI-kappaBalpha by BAY 11-7085 resulted in decreased Bcl-2 protein levels in neonatal lung homogenates and decreased cell viability in lung primary cultures after hyperoxic exposure. Furthermore, neonatal p50-null mutant (p50(-/-)) mice showed increased lung DNA degradation and decreased survival in hyperoxia...Continue Reading

References

May 1, 1980·The Journal of Clinical Investigation·L FrankD Massaro
Jul 10, 1980·The New England Journal of Medicine·S M Deneke, B L Fanburg
Oct 13, 1983·The New England Journal of Medicine·W B DavisR G Crystal
Feb 1, 1993·The Journal of Clinical Investigation·L B Clerch, D Massaro
Aug 15, 1997·The Journal of Biological Chemistry·Y LiS Horowitz
Oct 24, 1997·Photochemistry and Photobiology·C H ContagD A Benaron
Apr 16, 1998·The Journal of Clinical Investigation·P A DenneryK D Poss
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·F S LeeT Maniatis
Nov 5, 1998·The Journal of Clinical Investigation·J BohuslavR J Ulevitch
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·C BéraudP A Baeuerle
Apr 27, 2000·American Journal of Respiratory Cell and Molecular Biology·C Barazzone, C W White
Apr 27, 2000·American Journal of Respiratory Cell and Molecular Biology·N S WardJ A Elias
Jun 3, 2000·Annual Review of Immunology·M Karin, Y Ben-Neriah
Jun 10, 2000·European Journal of Biochemistry·B KaltschmidtM L Schmitz
Oct 18, 2000·The Journal of Biological Chemistry·W R FranekL L Mantell
May 4, 2001·Brain Research. Developmental Brain Research·N SimakajornboonD Gozal
Sep 22, 2001·The Journal of Biological Chemistry·J F KurlandR E Meyn
Feb 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Marie-Laure FrancoChristophe Delacourt
Sep 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·S Buckley, D Warburton
Jan 1, 2003·Pediatric Research·Phyllis A DenneryPamela A Rodgers

❮ Previous
Next ❯

Citations

Apr 24, 2007·Free Radical Biology & Medicine·Yuko AritaJonathan M Davis
Jul 5, 2013·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Lincoln S SmithThomas R Martin
May 17, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Guang YangPhyllis A Dennery
Aug 17, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jen-Ruey TangSteven H Abman
Mar 1, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Cristiana IosefCristina M Alvira
Dec 17, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Clyde J WrightPhyllis A Dennery
May 5, 2007·American Journal of Respiratory and Critical Care Medicine·Richard L AutenKathryn M Auten
Jan 27, 2007·American Journal of Respiratory and Critical Care Medicine·Cristina M AlviraMarlene Rabinovitch
Aug 8, 2009·American Journal of Respiratory and Critical Care Medicine·Stéphanie CarnesecchiConstance Barazzone Argiroffo
Apr 29, 2008·American Journal of Respiratory Cell and Molecular Biology·Vineet BhandariJack A Elias
Sep 9, 2008·American Journal of Respiratory Cell and Molecular Biology·Narasaiah Kolliputi, Aaron B Waxman
Jan 11, 2011·American Journal of Respiratory Cell and Molecular Biology·Anantha HarijithVineet Bhandari
May 23, 2003·American Journal of Respiratory Cell and Molecular Biology·Sandra PerkowskiSteven M Albelda
May 23, 2007·Pediatric Research·Cynthia MasalungaElsie S Mainali
Mar 17, 2009·Pediatric Research·Clyde J Wright, Phyllis A Dennery
Jan 3, 2020·The Journal of Clinical Investigation·Colleen M BartmanY S Prakash

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
electrophoretic mobility shift assay
FCS
electrophoresis
PCR

Software Mentioned

Living Image
StatView

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved