MavN is a Legionella pneumophila vacuole-associated protein required for efficient iron acquisition during intracellular growth

Proceedings of the National Academy of Sciences of the United States of America
Dervla T IsaacRalph R Isberg

Abstract

Iron is essential for the growth and virulence of most intravacuolar pathogens. The mechanisms by which microbes bypass host iron restriction to gain access to this metal across the host vacuolar membrane are poorly characterized. In this work, we identify a unique intracellular iron acquisition strategy used by Legionella pneumophila. The bacterial Icm/Dot (intracellular multiplication/defect in organelle trafficking) type IV secretion system targets the bacterial-derived MavN (more regions allowing vacuolar colocalization N) protein to the surface of the Legionella-containing vacuole where this putative transmembrane protein facilitates intravacuolar iron acquisition. The ΔmavN mutant exhibits a transcriptional iron-starvation signature before its growth is arrested during the very early stages of macrophage infection. This intracellular growth defect is rescued only by the addition of excess exogenous iron to the culture medium and not a variety of other metals. Consistent with MavN being a translocated substrate that plays an exclusive role during intracellular growth, the mutant shows no defect for growth in broth culture, even under severe iron-limiting conditions. Putative iron-binding residues within the MavN protein we...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A MarraH A Shuman
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J van RenswoudeR D Klausner
Jan 1, 1983·Annual Review of Microbiology·W Harder, L Dijkhuizen
Sep 1, 1995·Infection and Immunity·M S Swanson, R R Isberg
May 27, 1994·Gene·E K Hickey, N P Cianciotto
Oct 23, 1997·International Journal of Systematic Bacteriology·V RouxD Raoult
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·J J De VossC E Barry
Oct 29, 2000·Cell·M M WöstenE A Groisman
Feb 8, 2002·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·L A Gaither, D J Eide
Sep 14, 2002·Infection and Immunity·Marianne Robey, Nicholas P Cianciotto
Jan 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhao-Qing Luo, Ralph R Isberg
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jayong ChungMarianne Wessling-Resnick
Jul 1, 2004·Molecular Microbiology·Ari B Molofsky, Michele S Swanson
Sep 28, 2004·Science·Minchen ChienJames J Russo
Dec 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hiroki NagaiCraig R Roy
Jan 18, 2005·FEMS Microbiology Reviews·Gil SegalTal Zusman
Feb 3, 2006·Journal of Bacteriology·Kimberly A AllardNicholas P Cianciotto
Aug 31, 2006·The Journal of Experimental Medicine·Vicki P Losick, Ralph R Isberg
Nov 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rita K LagunaRalph R Isberg
Nov 18, 2008·Nature Reviews. Microbiology·Ralph R IsbergMatthew Heidtman
Apr 29, 2009·Infection and Immunity·Kimberly A AllardNicholas P Cianciotto
May 12, 2009·Nucleic Acids Research·Andreas BernselArne Elofsson

❮ Previous
Next ❯

Citations

Jul 18, 2017·Nature Reviews. Microbiology·Jiazhang Qiu, Zhao-Qing Luo
Feb 7, 2018·Frontiers in Cellular and Infection Microbiology·Giulia OlivaCarmen Buchrieser
Dec 1, 2017·Frontiers in Cellular and Infection Microbiology·Soma Ghosh, Tamara J O'Connor
Nov 15, 2017·Proceedings of the National Academy of Sciences of the United States of America·Stephanie R ShamesCraig R Roy
May 18, 2016·Infection and Immunity·Tamara J O'ConnorRalph R Isberg
Aug 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Eric T ChristensonAnirban Banerjee
Mar 22, 2016·Current Opinion in Infectious Diseases·Elizabeth Ann Misch
Mar 3, 2020·Frontiers in Immunology·A Leoni Swart, Hubert Hilbi
Oct 19, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Elif Özlem Arslan-Aydoğdu, Ayten Kimiran
Sep 26, 2017·Frontiers in Cellular and Infection Microbiology·Samual C AllgoodM Ramona Neunuebel
Jul 30, 2019·BioMed Research International·Takashi NishidaMasahisa Watarai
Feb 6, 2021·Communications Biology·Nicole A EllisMatthias P Machner
Apr 13, 2021·Virulence·Deepika Chauhan, Stephanie R Shames
Jun 23, 2021·MSystems·Michael SweetRaquel Peixoto
Aug 9, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Francisco Amaro, Ana Martín-González

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Elizabeth A Creasey, Ralph R Isberg
Annual Review of Cell and Developmental Biology
Andree Hubber, Craig R Roy
Advances in Experimental Medicine and Biology
Justin A McDonoughCraig R Roy
© 2021 Meta ULC. All rights reserved