PMID: 377231Jan 1, 1979Paper

Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin-Dahlem, GFR

Nucleic Acids Research
C Zwieb, R Brimacombe

Abstract

It is well established that when E. coli 30S ribosomal subunits are irradiated with ultraviolet light under mild conditions a specific cross-link is formed between protein S7 and the 16S RNA. Methodology is presented for the analysis of the single nucleotide residue concerned in this cross-link. Firstly, the identity of the ribonuclease T1 octanucleotide attached to S7 is confirmed by a new method, which involves isolation and analysis of S7-polynucleotide complexes containing 30 -- 40 nucleotides. Secondly, the isolated S7-octanucleotide complex is digested successively with ribonuclease A, proteinase K and ribonuclease T2, and the nucleotides liberated are identified. The results show unambiguously that uridine residue number 1239 in the 16S RNA sequence is cross-linked to protein S7.

Citations

Jan 1, 1993·Molecular & General Genetics : MGG·D Zhuo, L Bonen
Sep 22, 1982·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H G Wittmann
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·Y G ChuC R Cantor
Feb 1, 1984·Photochemistry and Photobiology·M D ShetlarK Hom
Nov 1, 1980·European Journal of Biochemistry·A J WagenmakersW J van Venrooij
Sep 22, 1980·FEBS Letters·C Chiarrutini, A Expert-Bezançon
Jan 20, 1981·Molecular and Cellular Biochemistry·G R KunkelH G Martinson
Jan 1, 1986·Progress in Biophysics and Molecular Biology·K Nagano, M Harel
Jan 1, 1982·Progress in Biophysics and Molecular Biology·C Hélène, G Lancelot
Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
Jul 5, 1985·Journal of Molecular Biology·A Expert-Bezançon, P L Wollenzien
Nov 20, 1988·Journal of Molecular Biology·S SternH F Noller
Oct 3, 1985·Biochimica Et Biophysica Acta·H A RauéR J Planta
Sep 17, 1988·Journal of Theoretical Biology·K NaganoM Takezawa
Apr 24, 1981·Science·H F Noller, C R Woese

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