PMID: 9171388Jun 1, 1997Paper

Maximization of transcription of the serC (pdxF)-aroA multifunctional operon by antagonistic effects of the cyclic AMP (cAMP) receptor protein-cAMP complex and Lrp global regulators of Escherichia coli K-12

Journal of Bacteriology
T K ManM E Winkler

Abstract

The arrangement of the Escherichia coli serC (pdxF) and aroA genes into a cotranscribed multifunctional operon allows coregulation of two enzymes required for the biosynthesis of L-serine, pyridoxal 5'-phosphate, chorismate, and the aromatic amino acids and vitamins. RNase T2 protection assays revealed two major transcripts that were initiated from a promoter upstream from serC (pdxF). Between 80 to 90% of serC (pdxF) transcripts were present in single-gene mRNA molecules that likely arose by Rho-independent termination between serC (pdxF) and aroA. serC (pdxF)-aroA cotranscripts terminated at another Rho-independent terminator near the end of aroA. We studied operon regulation by determining differential rates of beta-galactosidase synthesis in a merodiploid strain carrying a single-copy lambda[phi(serC [pdxF]'-lacZYA)] operon fusion. serC (pdxF) transcription was greatest in bacteria growing in minimal salts-glucose medium (MMGlu) and was reduced in minimal salts-glycerol medium, enriched MMGlu, and LB medium. serC (pdxF) transcription was increased in cya or crp mutants compared to their cya+ crp+ parent in MMGlu or LB medium. In contrast, serC (pdxF) transcription decreased in an lrp mutant compared to its lrp+ parent in MM...Continue Reading

References

Sep 1, 1976·Bacteriological Reviews·I Pastan, S Adhya
May 16, 1990·Biochemical and Biophysical Research Communications·H HamaT Tsuchiya
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C G Cupples, J H Miller
Jul 1, 1985·Journal of Bacteriology·S K Hoiseth, B A Stocker
Dec 1, 1968·Journal of Molecular Biology·D E Morse, C Yanofsky
Aug 28, 1969·Journal of Molecular Biology·D E Morse, C Yanofsky
Sep 1, 1995·Journal of Bacteriology·Y CuiJ M Calvo
Dec 25, 1994·Nucleic Acids Research·B S PowellC L Turnbough
Jun 21, 1994·Biochimica Et Biophysica Acta·C J LimJ A Fuchs
Jan 1, 1993·Annual Review of Biochemistry·A KolbS Adhya
Jan 1, 1995·Annual Review of Microbiology·E B Newman, R Lin
Jan 1, 1996·Nucleic Acids Research·M Kröger, R Wahl
Dec 1, 1996·Journal of Bacteriology·J R LandgrafJ M Calvo
Dec 1, 1996·Journal of Bacteriology·E MathewM Freundlich
Sep 1, 1964·Journal of Bacteriology·L I PIZER, M L POTOCHNY

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Citations

May 3, 2002·Molecular Microbiology·Li YangElaine B Newman
Feb 3, 2006·Journal of Bacteriology·Rita M CornishC Dale Poulter
Feb 15, 2002·Journal of Bacteriology·Andrew J PeaseMalcolm E Winkler
Dec 1, 2004·EcoSal Plus·George V Stauffer
Apr 1, 2021·EcoSal Plus·Angela TramontiRoberto Contestabile
Sep 1, 2008·EcoSal Plus·James Pittard, Ji Yang
Jan 26, 2017·ACS Synthetic Biology·Yongfei LiuDawei Zhang

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