Maximizing the adequacy of Hologic(®) Cervista(®) HPV HR results on ThinPrep(®) Pap samples treated with glacial acetic acid

Diagnostic Cytopathology
Aparna MahajanSuzanne M Selvaggi

Abstract

Although glacial acetic acid (GAA) treatment of bloody cervical samples has reduced the rate of unsatisfactory Pap Tests, recent studies suggest that it may negatively impact high-risk (hr)-HPV test results. The objectives of this study were to compare the levels of genomic DNA between GAA treated and nontreated ThinPrep(®) samples using the Hologic(®) Cervista(®) HPV-HR assay and to compare the adequacy of the ThinPrep(®) Pap Test between aliquoted and nonaliquoted samples. Prior to GAA treatment, 2.5 ml of the cervical sample was prealiquoted from 102 bloody ThinPrep(®) vials. Both GAA treated and nontreated samples were analyzed for hr-HPV using the Cervista(®) HPV HR assay. The levels of genomic DNA were measured and compared between these samples. In addition, ThinPrep(®) Pap Test adequacy rates were calculated and compared on aliquoted and nonaliquoted cervical samples. Of the 102 cervical samples, 95 (93%) nontreated aliquots contained satisfactory levels of genomic DNA as compared to 36 (35%) GAA-treated samples (p < 0.00001). Ninety-nine (97%) aliquoted cervical samples were satisfactory for cytologic evaluation as compared to 1,326 (96%) GAA treated samples from 2013 (nonaliquoted); not statistically significant (p = ...Continue Reading

References

Mar 13, 2002·American Journal of Clinical Pathology·Joel S BentzC Jay Marshall
Feb 14, 2006·Diagnostic Cytopathology·Lori A HaackSuzanne M Selvaggi
Aug 11, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Janel DockterCristina Giachetti
Feb 6, 2013·Cytopathology : Official Journal of the British Society for Clinical Cytology·C MooreH A Cubie
Mar 27, 2013·Journal of Lower Genital Tract Disease·L Stewart MassadUNKNOWN 2012 ASCCP Consensus Guidelines Conference

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Citations

Oct 27, 2018·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Carolann RisleyStephen S Raab

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