PMID: 6410396Aug 1, 1983Paper

Maximum-entropy calculation of the electron density at 4 A resolution of Pf1 filamentous bacteriophage

Proceedings of the National Academy of Sciences of the United States of America
R K BryanD A Marvin

Abstract

A 4 A electron-density map of Pf1 filamentous bacterial virus has been calculated from x-ray fiber diffraction data by using the maximum-entropy method. This method produces a map that is free of features due to noise in the data and enables incomplete isomorphous-derivative phase information to be supplemented by information about the nature of the solution. The map shows gently curved (banana-shaped) rods of density about 70 A long, oriented roughly parallel to the virion axis but slewing by about 1/6th turn while running from a radius of 28 A to one of 13 A. Within these rods, there is a helical periodicity with a pitch of 5 to 6 A. We interpret these rods to be the helical subunits of the virion. The position of strongly diffracted intensity on the x-ray fiber pattern shows that the basic helix of the virion is right handed and that neighboring nearly parallel protein helices cross one another in an unusual negative sense.

References

Nov 30, 1976·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D A Marvin, E J Wachtel
Jan 3, 1975·Nature·D A Marvin, E J Wachtel
Mar 20, 1975·Nature·K C HolmesU Gallwitz
Apr 1, 1972·Journal of the Optical Society of America·B R Frieden
Jan 5, 1981·Journal of Molecular Biology·C ChothiaD Richardson

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Citations

Sep 15, 1989·Experientia·J W Galloway
Oct 19, 2007·European Biophysics Journal : EBJ·S K StrausD A Marvin
Apr 19, 2008·European Biophysics Journal : EBJ·S K StrausD A Marvin
Nov 18, 2010·European Biophysics Journal : EBJ·S K StrausD A Marvin
Apr 22, 2004·Nature Reviews. Microbiology·Lisa CraigJohn A Tainer
Mar 4, 2014·Progress in Biophysics and Molecular Biology·D A MarvinS K Straus
Jun 1, 1989·International Journal of Biological Macromolecules·D A Marvin
Dec 15, 2016·Physical Review. E·Carlo Cafaro, Sean Alan Ali

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