Apr 20, 2020

Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka, Oryzias latipes

BioRxiv : the Preprint Server for Biology
Y. Kato-UnokiYuji Oshima

Abstract

Tributyltin-binding proteins (TBT-bps), members of the lipocalin family, bind TBT in fish blood and are presumed to contribute to detoxification of TBT. Recent studies have shown that many fish species have TBT-bp genes, and that these genes are induced by stresses such as exposure to chemicals or fish pathogenic bacteria. However, the function of TBT-bps, and the mechanisms of their induction and detoxification activity are still unclear. Here, towards elucidating the functions of TBT-bp2, we produced a TBT-bp2 knockout (TBT-bp2-/-) strain of Japanese medaka, Oryzias latipes, by using the CRISPR/Cas9 system. Gene expression of the mutated TBT-bp2 was reduced, and the cDNA sequencing and predicted protein structure suggested possible loss of function. However, the fish could be grown under normal conditions. Exposure of the TBT-bp2-/- strain of medaka to various stresses in future experiments is expected to contribute to our understanding of this novel detoxification system in aquatic organisms.

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Mentioned in this Paper

Metabolic Process, Cellular
Genes
Toxin
NF-kappaB p50/p65 Complex
RELA gene
Apoptosis
Analogs & derivatives
Escherichia coli
Cell Death
Genes, Overlapping

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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