MBD2 regulates differentiation and function of Th17 cells in neutrophils- dominant asthma via HIF-1α

Journal of Inflammation
Li XuXu D Xiang

Abstract

T helper 17 (Th17) cells have proven to be crucial in the pathogenesis of neutrophils-dominant asthma. Hypoxia inducible factor-1α (HIF-1α) is involved in allergic responses in asthma. Our previous studies indicated that Methtyl-CpG binding domain protein 2 (MBD2) expression was increased in asthma patients. The aim of the present study is to understand how MBD2 interacts with HIF-1α to regulate Th17 cell differentiation and IL-17 expression in neutrophils-dominant asthma. A neutrophils-dominant asthma mouse model was established using female C57BL/6 mice to investigate Th17 cell differentiation and MBD2 and HIF-1α expression regulation using flow cytometry, western blot or qRT-PCR. MBD2 and HIF-1α genes were silenced or overexpressed through lentiviral transduction to explore the roles of MBD2 in Th17 cell differentiation and IL-17 release in neutrophils-dominant asthma. A neutrophilic inflammatory asthma phenotype model was established successfully. This was characterized by airway hyperresponsiveness (AHR), increased BALF neutrophil granulocytes, activated Th17 cell differentiation, and high IL-17 levels. MBD2 and HIF-1α expression were significantly increased in the lung and spleen cells of mice with neutrophils-dominant as...Continue Reading

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Citations

Nov 7, 2019·Current Opinion in Allergy and Clinical Immunology·Xuesong ChenEvan Li
Feb 10, 2019·Biological Trace Element Research·Qiaojian ZhangShiwen Xu
Nov 17, 2019·Genes·Andrée-Anne Hudon Thibeault, Catherine Laprise
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Jan 22, 2022·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiqiang LiuBing Xiao

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Methods Mentioned

BETA
transfection
Reverse Transcription PCR
flow cytometry
ELISA
histone acetylation

Software Mentioned

SPSS
FACSCalibur
FlowJo

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