MBP-1 inhibits breast cancer growth and metastasis in immunocompetent mice

Cancer Research
Tatsuo KandaRatna B Ray

Abstract

Breast cancer is the leading cause of cancer death among women. We have shown previously an antiproliferative effect of MBP-1 on several human cancer cells. In this study, we have examined the potential of MBP-1 as a gene therapeutic candidate in regression of breast cancer growth and metastasis in an immunocompetent mouse model. For this, we have used a mouse breast cancer cell line (EO771) and syngeneic C57BL/6 mice. EO771 cells were implanted into the mammary fat pad of C57BL/6 mice. Replication-deficient recombinant adenovirus expressing MBP-1 was administered intratumorally to determine gene therapeutic potential. The results showed a significant regression of primary and distant (lung) tumor growth. Animals exhibited prolonged survival on treatment with MBP-1 compared with the control group (dl312). Subsequent studies suggested that MBP-1 inhibits matrix metalloproteinase expression in human breast cancer cells. Cells transduced with MBP-1 displayed inhibition of migration in a wound-healing assay. The conditioned medium from MBP-1-transduced cells blocked in vitro tube formation assay and inhibited expression of several angiogenic molecules. Taken together, our study shows that MBP-1 acts as a double-edged sword by inhib...Continue Reading

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Citations

Aug 29, 2013·International Journal of Cancer. Journal International Du Cancer·Hong LinZhenqing Feng
Dec 7, 2018·International Journal of Molecular Sciences·Zijin LiuGenfa Zhang
May 21, 2019·Frontiers in Cell and Developmental Biology·Miroslava DidiasovaMalgorzata Wygrecka

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