Abstract
Zebrafish rapidly alter their pigmentation in response to environmental changes. For black melanocytes, this change is due to aggregation or dispersion of melanin in the cell. Dispersion and aggregation are controlled by intracellular cyclic adenosine monophosphate (cAMP) levels, which increase upon stimulation by alpha melanocyte-stimulating hormone (alpha-MSH) or reduce with melanin-concentrating hormone (MCH). In mammals and birds, the melanocortin-1-receptor (MC1R) responds to MSH, and stimulates the synthesis of black eumelanin. While MSH-cAMP signaling stimulates melanogenesis in mammals, and melanosome dispersal in cold-blood vertebrates, the pathway components are highly conserved. However, it has only been assumed that mc1r mediates melanosome dispersal in fish. Here, using morpholino oligonucleotides designed to knockdown mc1r expression, we find that mc1r morphants are unable to disperse melanosomes when grown in dark conditions. We also use chemical modifiers of the cAMP pathway, and find an unexpected response to the specific phosphodiesterase 4 (PDE4) inhibitor, rolipram, in melanosome dispersal. When treated with the drug, melanosomes fail to fully disperse in dark conditions, despite presumed increased levels of...Continue Reading
References
Sep 14, 1992·FEBS Letters·V Chhajlani, J E Wikberg
Nov 1, 1995·Nature Genetics·P ValverdeA J Thody
Oct 27, 1994·Nature·D LuW O Wilkison
Jun 1, 1997·The EMBO Journal·C SakaiV J Hearing
Jan 1, 1997·Human Molecular Genetics·I J Jackson
Feb 28, 1998·Genes & Development·M M OllmannG S Barsh
Oct 13, 2000·Human Molecular Genetics·N FlanaganJ L Rees
Oct 21, 2000·Pigment Cell Research·R Fujii
Nov 2, 2001·Human Molecular Genetics·E HealyI J Jackson
Jan 11, 2002·Developmental Biology·J F RawlsS L Johnson
Feb 23, 2002·Trends in Genetics : TIG·Eve M Mellgren, Stephen L Johnson
Sep 21, 2002·Microscopy Research and Technique·James A Lister
Mar 7, 2003·Genomics·Darren W LoganIan J Jackson
Oct 25, 2003·Cellular Signalling·Tony P M AnderssonSamuel P S Svensson
Dec 19, 2003·Current Biology : CB·David M Parichy
Jul 15, 2004·Pigment Cell Research·Robert N Kelsh
Sep 17, 2004·American Journal of Human Genetics·Jonathan L Rees
Feb 8, 2005·Current Biology : CB·E Elizabeth PattonLeonard I Zon
Mar 12, 2005·Pigment Cell Research·M Lynn LamoreuxKenjiro Ozato
Jun 24, 2005·Human Molecular Genetics·Kimberley A BeaumontRichard A Sturm
Nov 18, 2005·General and Comparative Endocrinology·Akiyoshi Takahashi, Hiroshi Kawauchi
Dec 17, 2005·Science·Rebecca L LamasonKeith C Cheng
May 18, 2006·Pigment Cell Research·Darren W LoganIan J Jackson
Jul 1, 2006·Science·Maria Teresa LandiBoris C Bastian
Aug 2, 2006·Molecular BioSystems·Da-Woon JungSeth J Orlow
Aug 3, 2006·Nature Reviews. Drug Discovery·Frank S MennitiChristopher J Schmidt
Aug 18, 2006·Development·Chao-Tsung Yang, Stephen L Johnson
Sep 22, 2006·Nature·John A D'OrazioDavid E Fisher
Feb 23, 2007·Nature·Jennifer Y Lin, David E Fisher
Mar 14, 2007·Cell·Rutao CuiDavid E Fisher
Mar 21, 2007·Pigment Cell Research·Tae-Young ChoiTae-Jin Yoon
Jun 15, 2007·The Journal of Investigative Dermatology·Li Ni-Komatsu, Seth J Orlow
Jun 22, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jonathan D BestAlan G Roach
Oct 9, 2007·Current Biology : CB·Lavinia SheetsBruce J Schnapp
Mar 28, 2008·International Journal of Cancer. Journal International Du Cancer·Sara RaimondiMaria Concetta Fargnoli
Citations
May 8, 2010·Journal of Molecular Evolution·Frederico HenningAxel Meyer
Jan 6, 2010·Zebrafish·Zhiqiang ZengE Elizabeth Patton
Jan 19, 2012·Pigment Cell & Melanoma Research·Sarah ColanesiE Elizabeth Patton
Sep 13, 2011·PLoS Genetics·Emma R GreenhillRobert N Kelsh
Dec 21, 2010·PloS One·Cristina SantorielloMarina Mione
Feb 6, 2014·PloS One·Hong LiuFuren Zhang
Mar 8, 2014·PloS One·Chenghui WangGuoqing Lu
Jul 7, 2011·International Journal of Molecular Sciences·Charles H Williams, Charles C Hong
Oct 13, 2012·Journal of Cosmetic and Laser Therapy : Official Publication of the European Society for Laser Dermatology·Jae Hwan KimIl-Hwan Kim
Sep 29, 2011·Molecular and Cellular Endocrinology·Sarah M Tuziak, Hélène Volkoff
Jan 12, 2011·Biochemical and Biophysical Research Communications·Jian Xu, Fu-kang Xie
Jun 23, 2009·General and Comparative Endocrinology·E SánchezJ M Cerdá-Reverter
Apr 8, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Seungwoo JungTeresa M Gunn
Jan 13, 2011·Cellular & Molecular Biology Letters·Saima Salim, Sharique A Ali
Jun 17, 2015·Pigment Cell & Melanoma Research·Verena A KottlerChristine Dreyer
Mar 21, 2017·Scientific Reports·Inbal ShainerYoav Gothilf
Apr 6, 2017·Pigment Cell & Melanoma Research·Ellen van RooijenLeonard I Zon
Feb 9, 2018·Molecular Ecology·Jonathan L WhitneyStephen A Karl
Feb 23, 2019·Pigment Cell & Melanoma Research·Gabriel E BertolesiSarah McFarlane
Jan 10, 2018·The EMBO Journal·Rajender K MotianiRajesh S Gokhale
Oct 20, 2018·International Journal of Molecular Sciences·Yu-Mi KimYoung-Kwon Seo
Oct 23, 2018·Medicina·Ahmad Firdaus B Lajis
Jun 20, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Soon-Ho YimDarren R Williams
Feb 11, 2020·Stress : the International Journal on the Biology of Stress·Konstantin A DeminAllan V Kalueff
Mar 3, 2020·Frontiers in Cell and Developmental Biology·Yipeng LiangClaudius F Kratochwil
Dec 11, 2020·PLoS Genetics·Romain MadelainePhilippe Mourrain
Jun 21, 2021·EvoDevo·Marleen KlannVincent Laudet
Nov 3, 2017·Journal of Medicinal Chemistry·Yang ZhouMinying Cai