PMID: 26339380Sep 5, 2015Paper

MCI extraction from Turkish galls played protective roles against X-ray-induced damage in AHH-1 cells

International Journal of Clinical and Experimental Pathology
Jianhua YangJunping Hu

Abstract

To investigate the protective effects of MCI extract from Turkish galls against apoptosis induced by X-ray radiation in the AHH-1. The cells were divided into: control group; X-ray radiation group; MCI group, in which the confluent cells were preincubated with 5 μg/ml MCI for 2 h followed by radiation. For the radiation, cells preincubated with MCI were exposed to X-ray beams with a dose of 8 Gy in total. Cell viability, apoptosis and intracellular alteration of redox were monitored by MTT and flow cytometry. Compared with radiation group, the number of cells arrested at the G0/G1 phase was significantly reduced in MCI group (P < 0.05). X-ray radiation induces remarkable apoptosis in AHH-1, which was reversed by MCI. Compared with the radiation group, the generation of intracellular reactive oxygen species (ROS) was abrogated by pre-incubation with MCI (P < 0.05). In addition, the up-regulation of procaspase-3 induced by radiation was reversed by MCI. Radiation could induce up-regulation of Bax and down-regulation of Bcl-2; however, it is reversed completely after administration of MCI. Further, the enhanced expression of ERK and JNK induced by radiation was reversed by MCI. MCI extract from Turkish galls played protective effe...Continue Reading

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