MCM3AP-AS1/miR-876-5p/WNT5A axis regulates the proliferation of prostate cancer cells

Cancer Cell International
Jie WuLi Zhang

Abstract

Although the fact that long non-coding RNA MCM3AP antisense RNA 1 (MCM3AP-AS1) is oncogenic in several cancers is well documented, very few researchers investigate its expression and function in prostate cancer. Paired prostate cancer samples were selected, and expressions of MCM3AP-AS1, miR-876-5p and WNT5A were examined by qRT-PCR. MCM3AP-AS1 shRNA was transfected into LNCaP and PC-3 cell lines, and then the proliferative activity and apoptosis of cancer cells were detected by CCK-8 assay, EdU assay and flow cytometry analysis, respectively. qRT-PCR and Western blot were used to analyze the changes of miR-876-5p and WNT5A. Luciferase reporter gene assay was employed to determine the regulatory relationship between miR-876-5p and MCM3AP-AS1, miR-876-5p and WNT5A. MCM3AP-AS1 was significantly up-regulated in cancerous tissues of prostate cancer samples, positively correlated with the expression of WNT5A, while negatively related with miR-876-5p. After transfection of MCM3AP-AS1 shRNA into prostate cancer cells, the proliferative ability of cancer cells was signally inhibited, but the apoptosis of cancer cells was increased. MCM3AP-AS1 shRNA could reduce the expression of WNT5A on both mRNA and protein levels. Besides, MCM3AP-AS...Continue Reading

References

Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Oct 15, 2005·Development·Ines Alvarez-Garcia, Eric A Miska
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Feb 19, 2011·Current Opinion in Genetics & Development·Ulf Andersson Ørom, Ramin Shiekhattar
Sep 6, 2014·Current Pharmaceutical Biotechnology·Yusuf Tutar
Apr 18, 2015·Journal of Experimental & Clinical Cancer Research : CR·Liang DengJi-Hong Zhang
Nov 28, 2015·Nucleic Acids Research·Maria D ParaskevopoulouArtemis G Hatzigeorgiou
Mar 2, 2016·Biochemical and Biophysical Research Communications·Jinjiang ChouTao Xi
Jun 18, 2017·Journal of Internal Medicine·P S Olofsson, K J Tracey
Jul 14, 2017·Cancer Research·Arunoday BhanSubhrangsu S Mandal
Sep 9, 2017·Cell Proliferation·Jinglin LiXingming Jiang
Jan 30, 2018·Frontiers in Molecular Neuroscience·Chunqing YangYunhui Liu
May 5, 2018·Pathology, Research and Practice·Yizhou WangRenyan Gong
Jul 15, 2018·Molecular Immunology·Kaili LiaoXiaozhong Wang
Jul 17, 2019·Journal of Cellular Physiology·Mingming WangXiangdong Li
Aug 2, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jixiang NiuFuzhou Li
Aug 4, 2019·Nature Communications·Jiayin TangJing-Yuan Fang
Aug 6, 2019·Frontiers in Endocrinology·Phoebe L SarkarColleen C Nelson

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Methods Mentioned

BETA
transfection
fluorescence microscopy
GTPase

Software Mentioned

TargteScan
SPSS13
TargetScan
Flowjo

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