PMID: 7543245Jul 1, 1995Paper

MCP-1-stimulated monocytes preferentially utilize beta 2-integrins to migrate on laminin and fibronectin

The American Journal of Physiology
T W PenberthyD T Graves

Abstract

Recruitment of monocytes to inflammatory sites involves a series of sequential attachments and detachments to extracellular matrix proteins in response to a chemoattractant gradient. In this study we compared the migration of human peripheral blood monocytes on different extracellular matrix proteins in response to monocyte chemoattractant protein-1 (MCP-1) and N-formylmethionyl-leucyl-phenylalanine. Monocytes migrated more effectively on laminin compared with other extracellular matrix proteins. In contrast, this preference was not observed with neutrophils, suggesting that the monocytes and neutrophils may have differences in their migration on extracellular matrix proteins. To study this further, function-blocking monoclonal antibodies were used to examine mechanistically whether beta 1- and beta 2-integrins were involved in monocyte migration on fibronectin or laminin in response to MCP-1. Monocyte migration on both laminin and fibronectin was blocked 100% (P < 0.05) by intact monoclonal antibody, F(ab') fragments, and F(ab')2 fragments to beta 2-integrins. We also determined that antibodies to beta 2-integrins block monocyte migration that has already been initiated. In contrast, antibody to the beta 1-integrins inhibited ...Continue Reading

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Citations

Aug 17, 1999·The Journal of Clinical Investigation·J TrialR D Rossen
Jan 5, 2007·Journal of Gastroenterology and Hepatology·Toshinori Kamisako, Hiroshi Ogawa
Mar 17, 2009·Cell Adhesion & Migration·Elena KostidouGeorge Koliakos
Jan 1, 1997·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·T W PenberthyD T Graves
Oct 25, 1996·Annals of the New York Academy of Sciences·J Gille, R A Swerlick
Sep 18, 2007·Journal of Neurochemistry·Nancy Nutile-McMenemyJoyce A Deleo
May 31, 2003·The Journal of Biological Chemistry·Jahangir IqbalM Mahmood Hussain
Nov 26, 2003·International Journal of Cancer. Journal International Du Cancer·Tobias SilzleLeoni A Kunz-Schughart
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C PedrazaM Patarroyo
Feb 1, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Steven J HenryDaniel A Hammer
Sep 27, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Stephen M HahnTheresa M Busch
Oct 12, 2004·The International Journal of Biochemistry & Cell Biology·Varvara TrachanaGeorge Koliakos

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