MD-2-dependent pulmonary immune responses to inhaled lipooligosaccharides: effect of acylation state.

American Journal of Respiratory Cell and Molecular Biology
Suzana HadinaPeter S Thorne

Abstract

Endotoxins represent one of the most potent classes of microbial immunoactive components that can cause pulmonary inflammation. The aim of this study was to compare the inflammatory potency of two types of Neisseria meningitidis endotoxins (lipooligosaccharides) in lungs: wild type (hexaacylated, LOS(wt)) and mutant type (pentaacylated, LOS(msbB)), and to determine the importance of MD-2 in endotoxin responses in lungs in vivo. Endotoxin-normoresponsive mice (BALB/c) were exposed to selected doses of penta- and hexaacylated lipooligosaccharides (LOS) by nasal aspiration. Cellular and cytokine/chemokine inflammatory responses in bronchoalveolar lavage were measured at 1-, 4-, 8-, 16-, 24-, and 48-hour time points. MD-2-null mice were exposed to one dose of hexaacylated LOS and inflammatory responses were measured after 4 and 24 hours. Inhalation of hexaacylated LOS resulted in strong inflammatory responses, while pentaacylated LOS was much less potent in inducing increases of neutrophils, TNF-alpha, macrophage inflammatory protein-1 alpha, IL-6, granulocyte colony-stimulating factor, and IL-1 beta concentration in bronchoalveolar lavage. Similar kinetics of inflammatory responses in lungs were found in both types of endotoxin ex...Continue Reading

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Feb 1, 2012·The Annals of Occupational Hygiene·Marie A CogginsPeter S Thorne
Jun 8, 2010·Applied and Environmental Microbiology·Peter S ThorneStephen J Reynolds
Mar 24, 2010·Environmental Health Perspectives·Sean SempleJon G Ayres
May 30, 2009·Environmental Health Perspectives·Peter S ThorneDarryl C Zeldin
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Aug 11, 2015·American Journal of Respiratory and Critical Care Medicine·Peter S ThorneDarryl C Zeldin
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Sep 5, 2014·The Annals of Occupational Hygiene·Brita Kilburg-BasnyatPeter S Thorne
Mar 27, 2018·Environmental Health Perspectives·Angelico MendyPeter S Thorne
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Aug 2, 2017·Environmental Health Perspectives·Jennifer C D'SouzaSara D Adar
May 12, 2012·American Journal of Industrial Medicine·Katarina KulhankovaPeter S Thorne
Jul 9, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Ulrike GehringBert Brunekreef
Dec 16, 2017·British Journal of Pharmacology·Yali ZhangGuang Liang
Nov 20, 2016·Journal of Cellular and Molecular Medicine·Yali ZhangGuang Liang
May 18, 2021·The Journal of Allergy and Clinical Immunology·Peter S Thorne

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