MdaB from Escherichia coli: cloning, purification, crystallization and preliminary X-ray analysis

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Melanie A AdamsZongchao Jia

Abstract

The gene mdaB from Escherichia coli encodes an enzyme with activity similar to that of mammalian DT-diaphorase. It has been reported that the protein is able to confer resistance to the antibiotics DMP 840, adriamycin and etoposide. The gene was cloned and overexpressed in E. coli, allowing purification of the protein to homogeneity. The protein co-purified with an unidentified flavin. Suitable crystals for X-ray diffraction experiments were obtained by hanging-drop vapour diffusion. Their space group was triclinic P1, with unit-cell parameters a = 48.664, b = 52.099, c = 86.584 A, alpha = 87.106, beta = 86.889, gamma = 63.526 degrees. X-ray diffraction data were collected to 2.5 A.

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Citations

Oct 12, 2021·Frontiers in Cellular and Infection Microbiology·Douglas J Fraser-PittDeborah A O'Neil

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