MDG-1, an Ophiopogon polysaccharide, regulate gut microbiota in high-fat diet-induced obese C57BL/6 mice

International Journal of Biological Macromolecules
Lin-lin ShiYi Feng

Abstract

Most plant polysaccharides cannot be digested and utilized by host enzymes, and must be subjected to microbial fermentation before being assimilated by the host. MDG-1, a water-soluble β-d-fructan extracted from the roots of Ophiopogon japonicus, has potent anti-obesity and hypoglycemic effects. Interestingly, we found that MDG-1 is hardly absorbed into the blood. We presumed that MDG-1 might exhibit its potent efficacy via regulating the gut microbiota of the host. However, the overall microbiota structure variation of obese mice treated with MDG-1 and the direct metabolic consequences of MDG-1 on specific microbiota phyla remain poorly understood. Here, obese male C57BL/6 mice induced by a high-fat diet were given either vehicle or MDG-1 at a dose of 300mg/kg for 12 weeks and the overall fecal gut microbiota structure change was analyzed via pyrosequencing. On this basis, we further separated and identified the dominant bacteria of the feces from the MDG-1 treated mice. These bacteria were then cultured with MDG-1 in vitro and their metabolic profiles were analyzed via a metabonomic approach. The results showed that MDG-1 could decrease the ratio of Firmicutes/Bacteroidetes, adjust the abnormal gut microbiota to the normal st...Continue Reading

Citations

Jan 31, 2016·Journal of Ethnopharmacology·Min-Hui ChenYi-Tao Wang
Jan 3, 2016·Journal of Ethnopharmacology·Feng ChenNian-Kai Zeng
Nov 5, 2016·International Journal of Biological Macromolecules·Ning ZhuLeshuai W Zhang
Dec 10, 2016·Journal of Agricultural and Food Chemistry·Chuhan FuZhengquan Su
May 24, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Chuyue PanNa Lu
Oct 7, 2016·Metabolic Brain Disease·Cicely ProctorSiriporn C Chattipakorn
Aug 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Huai-You WangKarl W K Tsim
Sep 22, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Shi-Jun YueYu-Ping Tang
Feb 9, 2021·Briefings in Functional Genomics·Changlu QiLiang Cheng
Feb 25, 2021·The American Journal of Chinese Medicine·Xiao LiHaixue Kuang

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