Abstract
Esophageal cancer (EC) is one of the most common malignancies diagnosed in the Western world with an increasing incidence noted for esophageal adenocarcinoma (EAC). Despite improvements in staging, surgical procedures and postoperative treatments, the overall survival of patients with EC remains low. Murine double minute‑2 (MDM2) acts as an oncogene by inducing the degradation of the tumor‑suppressor protein TP53. In order to evaluate the MDM2 gene amplification status in EAC and squamous cell carcinoma (SCC), we established a quantitative PCR (qPCR) assay, screening a total of 127 esophageal carcinoma cases for MDM2 amplification. Esophageal carcinoma cases with enhanced MDM2 gene copy numbers were further analyzed by fluorescence in situ hybridisation (FISH) and MDM2 immunostaining. Among a total of 23 specimens (18%), identified by qPCR to possess elevated MDM2 gene copy numbers, one third (6.3%) showed a cluster‑like fluorescence pattern by FISH analyses and marked MDM2 protein immunostaining. MDM2 gene amplifications did not correlate with the occurrence of TP53 mutations. Due to the high therapeutic relevance of MDM2 overexpression, but the high cost of FISH, we suggest a primary screening of MDM2 copy number variations b...Continue Reading
References
Jun 1, 1995·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·A MarchettiG Bevilacqua
Jan 1, 1997·British Journal of Cancer·M HigashiyamaI Nishisho
Sep 27, 2000·International Journal of Cancer. Journal International Du Cancer·P TanièreP Hainaut
Jan 6, 2001·The American Journal of Pathology·P TanièreP Hainaut
Oct 26, 2005·Annals of Surgery·Paul M SchneiderArnulf H Hoelscher
Jan 29, 2008·The Annals of Thoracic Surgery·Arjun Pennathur, James D Luketich
Mar 4, 2008·Molecular Cancer Research : MCR·Ann ForslundPhilip B Paty
Jan 23, 2009·Cancer Investigation·C SengpielN Homann
Sep 17, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Daniel VallböhmerArnulf H Hölscher
Nov 4, 2009·Annals of Surgical Oncology·Tomoki MakinoYuichiro Doki
Dec 22, 2009·Future Oncology·Elfriede BollschweilerRalf Metzger
Jul 6, 2010·BMC Medical Genetics·Sabine Merkelbach-BruseEva Wardelmann
Nov 17, 2010·Annals of Surgical Oncology·Han-Shui HsuTzu-Hao Cheng
Apr 5, 2011·Journal of Surgical Oncology·Akinori EgashiraYoshihiko Maehara
Aug 11, 2012·Cancer Discovery·Nishant AgrawalStephen J Meltzer
Feb 5, 2013·Lancet·Arjun PennathurJames D Luketich
Jun 6, 2013·Journal of Gastroenterology and Hepatology·Daniel J RenoufGeoffrey Liu
Sep 17, 2013·World Journal of Gastroenterology : WJG·Yuwei Zhang
Nov 5, 2013·Current Medicinal Chemistry·S NagR Zhang
Dec 18, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Bo ChenMao-Ming Xiong
May 23, 2014·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·B ChenX-L Meng
Nov 15, 2014·Journal of Medicinal Chemistry·Yujun ZhaoShaomeng Wang
Jun 9, 2015·Oncotarget·Inga GrünewaldMarkus Stenner
Citations
Feb 11, 2018·Histopathology·Leora RobinsonPhilip Kaye
Oct 28, 2019·Combinatorial Chemistry & High Throughput Screening·Guobo DuBangxian Tan
Jul 12, 2020·Histopathology·Dongxian JiangYingyong Hou
Sep 22, 2018·Oncotarget·Vikas DemblaDavid S Hong
Aug 19, 2020·Cancers·Shomereeta RoyAntoine Italiano
Jul 5, 2017·Journal of Hematology & Oncology·Veronica TisatoGiorgio Zauli
Oct 31, 2019·Molecular Cancer Research : MCR·Daniel F ComiskeyDawn S Chandler
Apr 2, 2021·Cancer Management and Research·Juan YeRongzhang Xie
Jul 20, 2021·Pathology, Research and Practice·Jilong QinPeng Hou